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A Microtiter-Plate Assay of Human NOS Isoforms

 Time of Update: 2021-03-04

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NOS are heme proteins that also contain tightly bound flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) and require tetrahydrobiopterin (BH 4 ) for activity, whereas NADPH is used as a substrate.
Nucleic Acid Library Construction Using Synthetic DNA Constructs

 Time of Update: 2021-03-04

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This chapter outlines seven synthetic and molecular biology techniques that allow the controlled synthesis of nucleic acid libraries. Specifically: (1) The high-diversity chemical synthesis of point mutations; (2) the high-diversity chemical
Sci Adv: The control mechanism of the natural immune system

 Time of Update: 2021-03-04

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professor Steffen Thiel and student Rasmus Pihl, in the Department of Biomedical Research at Aolhus University, wanted to study which other proteins in our blood interact with so-called MASP proteases in the complement cascade reaction.
Nonradioactive Differential Display of Messenger RNA

 Time of Update: 2021-03-04

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Therefore, in the DD-PCR procedure mRNAs from different cell fractions are reverse transcribed (RT) using an oligo-dT-NN anchor primer.
In Vitro Continuation of RNA Synthesis Initiated in Vivo

 Time of Update: 2021-03-04

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Isolated nuclei will continue synthesis of RNA initiated in vivo, but reinitiation of synthesis is rare in washed nuclei ( 1 ).
Cell Stem Cell Interpretation! Scientists have developed a new DNA therapy that promises to remove cancer stem cells and successfully cure multiple myeloma

 Time of Update: 2021-03-04

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used ION251 to remove myeloma stem cells without damaging healthy blood cells. 'These preclinical results are so striking that half of the microscope images we compared bone marrow samples between treated mice and untreated mice have been blank
Koren's hanging up! Take 15 heavy-weight varieties, 36 over-rated varieties bright eyes, 11 new class 1 drugs, 41 new products on the road

 Time of Update: 2021-03-04

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Entering 2021, The Colum Pharmaceuticals product line continues to be happy news, 2 injections have been evaluated, 4 injections were approved at that time. just past 2020, Colum Pharmaceuticals has had a lot of gains: 15 varieties have been
Comey Pharmaceuticals' finance director was heavily fined

 Time of Update: 2021-03-04

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/> Although the risk of forced de-marketing is temporarily lifted, the risks faced by Comey Pharmaceuticals have not been completely eliminated! First, Kangmei Pharmaceuticals will face large-scale investor claims
Asymmetric Syntheses of Unnatural Amino Acids and Hydroxyethylene Peptide Isosteres

 Time of Update: 2021-03-04

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Chiral glycine equivalents serve as useful α-amino acid templates undergoing homologationvia carbon-carbon bond formation at the α-position through nucleophilic carbanion alkylation (2) or electrophilic carbocation substitution (3).
Radioligand Binding Methods for Membrane Preparations and Intact Cells

 Time of Update: 2021-03-04

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The radioligand binding assay is a relatively simple but powerful tool for studying G protein-coupled receptors.
Regret! Pharmaceutical giant Merck has become the first multinational drugmaker to announce it is abandoning research and development of the COVID-19 vaccine

 Time of Update: 2021-03-04

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In early 2020, the new coronary pneumonia (COVID-19) outbreak caused by the new coronavirus (SARS-CoV-2) spurred a major step forward in global drug and vaccine research and development, and in less than a year, new crown vaccines developed
Cryopreservation of Mammalian Semen

 Time of Update: 2021-03-04

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Mammalian spermatozoa were among the very first cells to be successfully cryopreserved and over the last five decades theuse of frozen-thawed semen for artificial insemination has come to play an important role in domestic livestock production.
Haizheng Pharmaceuticals gave a controlling stake in its subsidiary of $3.828 billion or eased its $10 billion debt

 Time of Update: 2021-03-04

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strains high-volume screening platform. At present, more than 20 biological pharmaceutical products, of which 9 products have received major new drug creation special support. Haizheng Bolly's 2015 listing of Anjuno is the first single-resistant
Chinese scholars have found that reprogramming T-cells enhances cancer immune efficacy

 Time of Update: 2021-03-04

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"Our goal is to improve the durability of tumor-specific T cells and their anti-tumor efficacy," said Dr. Chi Hongbo, a researcher in the Department of Immunology at St. Jude Children's Hospital and co-author of the paper.
The second round of volume procurement fire has been burned to self-cost medicine

 Time of Update: 2021-03-04

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In addition to Acapo sugar and solana, Adelfovesters, tadalaf and Allison Tan are all self-cost drugs, meaning that the war on volume purchases has spread above the price of medicines outside the National Health Insurance Directory.
Cell Rep: Painkillers can be used to repair spinal injuries

 Time of Update: 2021-03-04

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, including severe hypertension, slow heart rhythm and long-term immunosuppression. is a major problem in patients with spinal cord injury," said Popovich, a leading medical student. can lead to serious illnesses such as heart attacks
cDNA Library Construction Using Streptavidin-Paramagnetic Beads and PCR

 Time of Update: 2021-03-04

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c DNA clones of genes expressed in small amounts of material can be hard to obtain because the construction of conventionalcDNA libraries requires microgram amounts of poly (A) + RNA ( 1
A batch of ointments was exposed to strictly check the sale of non-drugs in pharmacies

 Time of Update: 2021-03-04

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　　It is reported that the Gusu District Market Supervision Bureau, after receiving the inspection recommendations, for retail pharmacies to carry out a special rectification activities.
Northern Blot Analysis

 Time of Update: 2021-03-04

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nucleic-acid probe, a techinque known as northern-blot analysis, is designed to address the study of RNA sequences1 This procedure, which derives from Southern-blot analysis (see Chapter 8 in this volume), is based on the ability of complementary
Understanding Asthma

 Time of Update: 2021-03-04

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During an attack, muscles surrounding the bronchial tubes tighten (bronchospasm), narrowing the air passage and interrupting the normal flow of air into and out of the lungs.

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