The author’s interest in microwave (MW) processing of biological samples for electron microscopy was initially stimulated by presentations and papers by Dr. Gary Login ( Login and Dvorak, 1988 ,Login and Dvorak, 1993 ,
Detection of autoantibodies to cytokines
Time of Update: 2020-11-24
Not only are in vitro neutralizing cytokine Ab not necessarily neutralizing in vivo, but assays for binding and neutralizing Ab to cytokines are often difficult to interpret.
Functional Glycomics and Glycobiology: An Overview
Time of Update: 2020-11-24
Glycomics is the study of the biological role of glycans and glycoconjugates, including glycoproteins, glycolipids, proteoglycans, and of protein–glycan interactions. This chapter outlines the scope of functional glycomics, from biological/biomedical significance to technology development.
Induction of Cytochrome P4501A (CYP1A) in Fish A Biomarker for Environmental Pollution
Time of Update: 2020-11-24
Inducible proteins are attractive candidates for biomarkers-i.e. biological responses that reflect exposure to, or effects of, environmental pollutants in an organism ( 1 ). Blomarkers are increasingly regarded as powerful and informative tools in ecotoxicology and environmental management ( 1 ).
Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with Contactless Conductivity Detection
Time of Update: 2020-11-24
Capillary electrophoresis (CE) with contactless conductivity detection (C 4 D) is readily applicable to determinations of amino acids in clinical samples.
Microwave Processing of Sediment Samples
Time of Update: 2020-11-24
Unfortunately, transmission electron microscope (TEM) sample preparation techniques are generally time-consuming processes requiring the replacement of pore fluid (seawater) with hydrophobic resin, through multiple serial dilutions.
Searching the Protein Interaction Space Through the MINT Database
Time of Update: 2020-11-24
Protein–protein interaction databases are thus becoming a major resource for investigating biological networks and pathways.
In this chapter we describe the Molecular INTeraction database (MINT).
Purification of Water-Soluble Natural Products
Time of Update: 2020-11-24
For example, a glance at the natural products section ofChemical Abstracts shows that about 95% of new substances reported are in the lipid-soluble category.
Biochemical determination of natural tumor-associated T-cell epitopes
Time of Update: 2020-11-23
We describe here a biochemical approach for the identification of tumor-associated T-cell epitopes.
Natural nitrogen fixation
Time of Update: 2020-11-22
, there is a root tumor bacteria in soybean plants, which contains nitrogen fixation enzymes that convert nitrogen into into nitrogen compounds .
Optimizing the Difference Gel Electrophoresis (DIGE) Technology
Time of Update: 2020-11-22
Difference gel electrophoresis (DIGE) technology has been used to provide a powerful quantitative component to proteomics experiments involving 2D gel electrophoresis. DIGE combines spectrally resolvable fluorescent dyes (Cy2, Cy3
X-Ray Microanalysis in the Scanning Electron Microscope
Time of Update: 2020-11-22
Aspects of qualitative and quantitative analysis are covered, including limitations of the technique.
Imaging Plant Nuclei and Membrane-Associated Cytoskeleton by Field Emission Scanning Electron Microscopy
Time of Update: 2020-11-21
This protocol shows how to isolate plant nuclei for SEM imaging of the nuclear envelope and associated structures from both sides of the nuclear envelope in cultured cells as well as in leaf or root cells.
