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Noninvasive Bioluminescent Imaging of Infections
Time of Update: 2021-02-28
This technology do not requires the sacrifice of the experimental animals, where the in vivo bioluminescence emissions in living animals permit the tracking of the infection.
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Phospholipases: An Overview
Time of Update: 2021-02-28
Although all phospholipases target phospholipids as substrates, they vary in the site of action on the phospholipids molecules, physiological function, mode of action, and their regulation.
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Applications of Tissue Microarray Technology
Time of Update: 2021-02-28
In combination with normal tissue arrays representing healthy tissues, prevalence, progression, and prognosis TMAs allow for a rapid and comprehensive analysis of molecular markers in human cancers.
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Capture of Intact Liposomes on Biacore Sensor Chips for ProteinMembrane Interaction Studies
Time of Update: 2021-02-28
This chapter describes the protocol for immobilization of liposomes on L1 sensor chips and discusses some of the experimental considerations.
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Mol Cell: Uncovering the special gene network of leukemia factors
Time of Update: 2021-02-28
'In this study, we designed a chemical genetic method that can shorten the analysis of transcription factors from the last few days to a few minutes,' said Dr. Kristy Stengel, a researcher at DOI:10.1016/j.molcel.2020.12.005.
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Nutritional Value of d-Amino Acids, d-Peptides, and Amino Acid Derivatives in Mice
Time of Update: 2021-02-28
The method is generally applicable to the determination of the biological utilization and safety of any amino acid derivative as a potential nutritional source of the correspondingl -amino acid.
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Post-electrophoretic identification of oxidized proteins
Time of Update: 2021-02-28
The optimal sequence and conditions for the staining process are (a) electrophoresis, (b) electroblotting onto PVDF membranes, (c) derivatization of carbonyls with 2,4-DNP, (d) immunostaining with anti DNP antibody, and (e) protein staining with colloidal gold.
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Baculovirus Vectors
Time of Update: 2021-02-28
formsof baculovlral DNA has greatly streainlined the lsolatlon and purlficatlon of a recoinblnant virus carrying the foreign geneof interest. This chapter summarize the wealth of transfer vectors and baculovnus DNAs available
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Reverse Transfection Using Gold Nanoparticles
Time of Update: 2021-02-28
In this manner, we constructed a method for reverse transfection using GC to deliver genes into the cells effectively.
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Scientists have revealed the mechanisms of a broad-spectrum anti-flu virus in ferro-based nanoenzymes
Time of Update: 2021-02-27
. Because influenza A virus has many subtypes and rapid variation characteristics, often lead to vaccine immune failure or drug resistance. Therefore, it is necessary to study and apply high-efficiency broad-spectrum influenza prevention and control
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Shotgun phage display Selection for bacterial receptins or other exported proteins
Time of Update: 2021-02-27
Shotgun phage display cloning involves construction of libraries from randomly fragmented bacterial chromosomal DNA , cloned genes, or eukaryoticcDNA s, into a phagemid vector.
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Automated Recombinant Protein Expression Screening in Escherichia coli
Time of Update: 2021-02-27
This chapter describes the automated procedure developed within the Structural Biology and Genomics Platform, Strasbourg for performing recombinant protein expression screening inEscherichia coli .
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Diagnosis of Krabbe Disease by Use of a Natural Substrate
Time of Update: 2021-02-27
To obviate this a modified assay is used where only the radiolabeled substrate is included in the incubation.
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Estimation of Chronological Age from the Racemization Rate of l- and d-Aspartic Acid: How to Completely Separate Enantiomers from Dentin
Time of Update: 2021-02-27
We describe the detailed procedures and the critical points for obtaining reliable estimated ages using the racemization method.
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Chinese scientists have discovered a new mechanism of "pathogenic protein" in Alzheimer's disease
Time of Update: 2021-02-27
at the University of Science and Technology of China, Professor Liu Qiang, found that this "pathogenic protein" inhibits the metabolism of neuron cells, revealing the underlying causes of learning and memory impairment in Alzheimer's patients. two
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Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes
Time of Update: 2021-02-27
Inspired by a natural fusion cytochrome P450 (P450) containing a monooxygenase domain and a reductasedomain, we have developed a heterotrimeric protein-utilized method to form a multienzyme complex composed of a bacterial P450and its catalytically essential two redox proteins.
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For the first time, animal experiments have separated "male and female sperm
Time of Update: 2021-02-27
For years, scientists have tried unsuccessfully to distinguish between these reproductive cells in different species because it would help in choosing the sex of animals and humans.
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Phosphopeptide mapping of proteins ectopically expressed in tissue culture cell lines
Time of Update: 2021-02-27
We generated phosphopeptide maps of the phosphorylated HAND1 using the methods described below and linked these modifications to changes in HAND1 biological function.
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Fluorescent Labeling of Proteins
Time of Update: 2021-02-27
Many single-molecule experimental techniques exploit fluorescence as a tool to investigate conformational dynamics and molecular interactions or track the movement of proteins in order to gain insight into their biological functions.
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Membrane Insertion of Small Proteins
Time of Update: 2021-02-27
The insertion of the protein into proteoliposomes can be followed by protease digestion and Western blot analysis.
