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Innovative technology EZ Clear: Transparent imaging of whole organs in mice
Time of Update: 2022-10-20
Wythe, PhD, of Baylor College of Medicine has developed an innovative technology called EZ Clear, a simple and fast new method that speeds up the process of presenting tissues as optically transparent, enabling 3D imaging of entire intact tissues and even entire organs in just three simple steps.
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Serious involution of PD-1
Time of Update: 2022-05-08
Activity data for the small molecule PD-L1 inhibitor INCB086550 Next, the researchers used a variety of antibodies to characterize the effect of INCB086550 on the formation and internalization of PD-L1 dimers .
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Monitoring Autophagy in Dictyostelium
Time of Update: 2021-02-19
Autophagy is an intracellular degradation mechanism essential for cell survival and maintenance of cellular homeostasis, differentiation, and development.
We describe here the use of confocal microscopy to detect the pattern of different autophagic markers and the differences expected in strains deficient in autophagy.
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Surface Plasmon Resonance Imaging Analysis of Protein Binding to a Sialoside-Based Carbohydrate Microarray
Time of Update: 2020-12-28
Contact printing of functional sialosides on neutravidin-coated surfaces was carried out and the properties of the resulting elements were characterized by fluorescence microscopy.
Sambucus nigra agglutinin (SNA) was used for testing on four different carbohydrate-functionalized surfaces and differential binding was analyzed.
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Large Multiprotein Structures Modeling and Simulation: The Need for Mesoscopic Models
Time of Update: 2020-12-26
Then we present 3DSPI, a model dedicated to studies of nuclear bodies based on a simple formalism inspired from molecular dynamics and coarse-grained models: particles interacting through a potential energy function and driven by an overdamped Langevin equation.
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Morphological Analysis of the Zebrafish Digestive System
Time of Update: 2020-12-13
The zebrafish provides an ideal model for the study of vertebrate organogenesis, including the formation of the digestive tract and its associated organs.
Despite optical transparency of embryos, the internal position of the developing digestive system and its close juxtaposition with the yolk initially made morphological analysis relatively challenging, particularly during the first 3 d of development.
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Fluorescence resonance energy transfer (FRET) as a method to calculate the dimerization strength of basic Helix-Loop-Helix (bHLH) proteins
Time of Update: 2020-12-05
In our study of the basic Helix-loop-Helix (bHLH) transcription factor HAND1, we show that HAND1 is phosphorylated during the trophoblast giant cell differentiation on residues residing in Helix I of the bHLH domain.
Our hypothesis is that these modifications result in changes in HAND1 dimerization affinities with other bHLH factors.
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FRET-Based Imaging of Rac and Cdc42 Activation During Fc-Receptor-Mediated Phagocytosis in Macrophages
Time of Update: 2020-11-22
Fluorescence resonance energy transfer (FRET) imaging can measure the spatial and temporal distributions of activated Rho GTPases within living cells.
This chapter describes FRET microscopy methods to image the distribution of GTP-bound Rac and Cdc42 during the well-defined morphological transitions of phagocytosis by macrophages.
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Time-Profiling Fluorescent Reporters in the Arabidopsis Root
Time of Update: 2020-11-20
Confocal laser scanning microscopy is a useful nondestructive approach for the visualization of fluorescent reportersin planta .
Here, we describe a technique to monitor changes in fluorescence in theArabidopsis root over a long period of time.
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Three-Dimensional Acrylamide Fluorescence In Situ Hybridization for Plant Cells
Time of Update: 2020-11-19
In particular, optical sectioning microscopy of fluorescence in situ hybridization (FISH)-stained nuclei provides three-dimensional spatial information about the organization and distribution of specific sequences and chromosomal loci within the nucleus.
Several examples illustrate the versatility of this technique for the investigation of meiotic telomere dynamics in maize,Arabidopsis , and oat.
