Dig Dis Sci: Spontaneous seroclearance is associated with a lower degree of liver fibrosis in patients with treatment-naïve chronic hepatitis B
Time of Update: 2022-11-25
This study confirms that spontaneous HBsAg serological clearance is still rare in patients with chronic HBV infection, but it is associated with low baseline HBsAg and lower levels of liver fibrosis detected by TE.
Research frontiers Safety outcomes of the TALAPRO-1 study
Time of Update: 2022-11-01
Fig. 1 Levels of TEAEs occurring in the safety population (A: Comprehensive; B: Hematology TEAEs; C: Non-hematology TEAEs)Hematology TEAEs usually occur in the 4-5 months of treatment start, the median duration of grade 3/4 hematology TEAEs such as anemia, neutropenia, and thrombocytopenia is usually 7-12 days, and patients do not experience grade 4 anemia or neutropenia events.
Front Immunol: Quantitative anti-HBc combined with quantitative HBsAg predicts HBsAg clearance in NA-suppressed chronic hepatitis B patients with PEG-IFN-a sequential therapy
Time of Update: 2022-09-30
The objective of this study was to investigate the dynamic changes in serum levels of q-anti-HBc and qHBcrAg in patients with chronic hepatitis B while receiving polyethylene glycolated interferon-a therapy, and to evaluate the role of these two biomarkers in predicting hepatitis B surface antigen clearance.
Multiple application scenarios of SNPs
Time of Update: 2022-08-30
SNPs also have other advantages, such as their abundance in all regions of the human genome and their suitability for analysis using high-throughput techniques, thus serving as surrogate identification markers for forensic analysis specifically targeting highly degraded DNA .
South China Botanical Garden reveals that chloroplast genome trees optimize the cognition of community construction in large forest plots in my country
Time of Update: 2022-01-08
A first-generation DNA barcode fragment ( matK + rbcL + trnH-psbA ) and 79 protein-coding genes of the chloroplast genome constructed a community phylogenetic tree, and compared the effects of different tree-building methods on the calculation results of phylogenetic diversity index and functional trait phylogenetic signals .
[Breakthrough] How terrible is chronic enteritis
Time of Update: 2021-11-15
A team from "Chronic Inflammation Precision Medicine" now shows that the risk gene XBP1 of chronic enteritis has a decisive influence on the way intestinal mucosal cells deal with DNA damage, thereby protecting themselves from the deterioration of cancer .
PNAS discovers a new mechanism and uses imaging technology to develop new sting-activating drugs
Time of Update: 2021-09-10
A new study by scientists at the Jonsson Comprehensive Cancer Center at UCLA has found that emerging drugs that activate the protein STING (a well-established regulator of immune cell activation) substantially alter the metabolism responsible for the building blocks of DNA nucleotides.
The latest article from Shanghai Jiaotong University: new discovery of spherical nucleic acid capturing miRNA in living cells
Time of Update: 2021-08-11
However, the application of miRNA sponge faces the challenge that the cell uptake efficiency and the ability to bind to the target nucleic acid in the cell cannot be balanced .
Programmable spherical nucleic acid, and use it for efficient capture of protocarcinoma miRNA in cells to inhibit tumor growth .
UV-Induced DNA Damage and DNA Repair in Ribosomal Genes Chromatin
Time of Update: 2021-03-02
nucleosomes rDNA, that the formation of CPDs promotes changes in the active rDNA chromatin, and that NER is coupled to rRNA genes transcription.
Agarose Gel Electrophoresis of Nucleic Acids
Time of Update: 2021-03-01
DNA fragments may be separated by gel electrophoresis in a gel composed of agarose.
This allows DNA fragments to be resolved on the basis of their molecular weight.
In general a 0.8–1% gel may be used for effective separation of DNA fragments of 100–1500 base pairs ( 1 ).
λ as a Cloning Vector
Time of Update: 2021-02-25
Genes can be identified and cloned by complementanon of mutants, hybrrdtzation of nucleic acids, screening for their products, or systematic sequencing of genetic enttties. The chotce of the strategy
Bioinformatics Techniques in Microarray Research: Applied Microarray Data Analysis Using R and SAS Software
Time of Update: 2021-02-18
DNA -microarray technology is a relatively recent and novel approach to conducting genome-wide gene expression studies to identify previously unknown biological pathways associated with disease.
This chapter outlines appropriate techniques for analyzing microarray data in a fashion that also yields a list of top genes with differential expression in a given experiment.
Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates
Time of Update: 2021-01-24
The method involves running a first-dimension gel in order to separate restriction-digested replication intermediates of different mass.
The 2-D gel is then blotted and probed to allow an examination of replication intermediates in specific DNA regions.
Time of Update: 2021-01-20
The term “agroinfection” was first used ( 1 ) to describe the use ofAgrobacterium for the introduction of infectious molecules to plants.
Replication of the agent within the plant often leads to systemic viral or viroidal symptoms that witness T- DNA transfer, independently of T-DNA integration.
Generation of Plant Small RNA cDNA Libraries for High-Throughput Sequencing
Time of Update: 2021-01-17
Conventional cloning and sequencing approaches have identified hundreds of miRNAs and a large number of siRNAs, but are no longer the best choices for identification of new miRNAs which are generally expressed at low abundance.