'Modular' super-enhancers control retinal development
Time of Update: 2022-01-24
Jude graduate student Victoria Honnell are part of a team that discovered distinct functions of super-enhancer regions that control gene expression during retinal formation .
Jude Children's Research Hospital studied the vx2 super-enhancer and its role in retinal development .
Cell: Subverting cognition
Time of Update: 2021-10-02
Using cryo-electron microscopy (cryo-EM) technology, this study proved that the viral histone doublets do indeed interact with DNA to form nucleosome-like particles, which have unique structural characteristics not seen in eukaryotes .
Preparation of DNA and RNA from Trypanosoma brucei
Time of Update: 2021-02-12
The use of VSGcDNA s to probe Southern blots of genomic DNA provided the demonstration that in most cases antigenic variation is the result of the duplicative transposition of a VSG gene to an expression site ( 6 – 8 ).
In Vivo Footprinting in Arabidopsis
Time of Update: 2021-01-18
The DNA/protein interactions targeted by m viva footprintmg can be visualized through the use of genomic sequencing, a technique that can provide strand-specific resolution of single bases ( 1 ).
High-Throughput TAIL-PCR as a Tool to Identify DNA Flanking Insertions
Time of Update: 2021-01-15
Thermal asymmetric interlaced polymerase chain reaction (TAIL- PCR ) is a fast and efficient method to amplify unknown sequences adjacent to known insertion sites inArabidopsis .
DNA Profiling: Theory and Practice
Time of Update: 2021-01-06
Of the vast range of techniques in use in modern molecular biology, DNA protiling is one of the more difficult to describe,primarily because the term “DNA Protiling” itself means very different thing
Reverse Genetics in Medicago truncatula Using Tnt1 Insertion Mutants
Time of Update: 2020-11-19
truncatula genome sequencing, availability of large-scale mutant populations becomes a priority.
Therefore, this PCR-based reverse screening is a rapid way of identifying knock-out mutants for specific genes inTnt1 -tagged population ofM.
One can search the database to find an insertion line for the gene of interest.
Nucleic Acid Extraction from Agrobacterium Strains
Time of Update: 2020-11-11
The successful use ofAgrobacterium as a tool for the genetic engineering of plant cells often requires the manipulation and analysis of nucleic acids present in recombinantAgrobacterium strains.
Here we present dependable methods for the isolation of genomic (total)DNA , mega-plasmid DNA, shuttle or binary plasmid DNA, and RNA.