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Mechanoregulation of the RNA-binding protein hnRNPC in a failed heart
Time of Update: 2023-01-05
Mechanical regulation helps clarify heart failuresummaryHeart disease is characterized by a strong remodeling of the extracellular matrix (ECM), which eventually leads to heart failure.
Mechanical regulation helps clarify heart failuresummaryHeart disease is characterized by a strong remodeling of the extracellular matrix (ECM), which eventually leads to heart failure.
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Differences between stromal cell subpopulations
Time of Update: 2022-10-03
Characterization of CD41+ cells in the lymph node Images of different segments of lymphatic vessels within mouse lymph nodes show that the vast majority of LYVE1+ cells co-express CD41.
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AOCC 2021 will start biologic therapy early to help achieve higher treatment goals for Crohn's disease
Time of Update: 2021-11-05
Subsequently, internationally renowned expert David Rubin and many domestic experts, combined with a number of real clinical cases, conducted wonderful discussions and exchanges on the treatment goals of IBD and the strategies for early use of biological agents .
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Immunity Tsinghua University's Dong Chen team discovered a new regulatory mechanism for T follicular helper cell development
Time of Update: 2021-10-21
The expression of transcription factor Zfp831 is inhibited by ERK, which promotes Tfh cell differentiation by directly up-regulating the expression of transcription factors Bcl6 and Tcf7 .
Therefore, this study determined that the ERK-Zfp831 axis, which is regulated by costimulatory signals, plays a key regulatory role in Tfh cell development .
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Nature: Chi Hongbo's team discovered a new pathway to regulate humoral immune response
Time of Update: 2021-08-11
In summary, this study identified a new pathway regulating the differentiation of TFH cells-CDP-ethanolamine pathway; it clarified that de novo PE synthesis signals determine the function of TFH cells and the strength of humoral immune response by regulating the location and stability of the chemokine receptor CXCR5 ; Reveal the importance of metabolic pathways and post-transcriptional regulation on the differentiation and fate of effector T cells .
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The Affiliated Hospital of Soochow University develops a new spray: to help heal the damage after a heart attack
Time of Update: 2021-07-29
Now, researchers report in the journal ACS Nano that they have developed a minimally invasive exosome spray that can help rats repair their hearts after myocardial infarction .
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New research reveals key regulators of muscle growth and is expected to develop new therapies for muscle injury
Time of Update: 2021-03-23
Muscle growth is regulated by Notch signaling pathwayTwo years ago, a research team led by Professor Carmen Birchmeier from the Max Delbrück Molecular Medicine Center (MDC) of the Helmholtz Federation in Berlin, Germany, showed that the process of stem cell development into myocytes is regulated by two proteins, Hes1 and MyoD.
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Ubiquitination of GPCRs
Time of Update: 2021-03-03
This involves co-expression of a GPCR with an epitope-tagged ubiquitin construct in a �heterologous mammalian expression system.
Stimulus-dependent modification of the GPCR by �ubiquitin is detected by immunoprecipitation and subsequent immunoblotting to detect incorporation of the epitope-tagged ubiquitin.
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FRET-Based Biosensors for the Detection and Quantification of AI-2 Class of Quorum Sensing Compounds
Time of Update: 2021-01-25
LuxP-BAI2 binding induces changes in fluorescence resonance energy transfer (FRET) between CFP and YFP, whose magnitude of change is ligand concentration dependent.
This report describes the use of the LuxP-FRET biosensor for the detection and quantification of BAI-2.
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Cell Death Dis: Blocking the hypusination effect of EIF5A inhibits the growth of colorectal cancer cells
Time of Update: 2020-12-31
when the cancer reaches advanced stage, the cancer cells move from place to place, resulting in poor prognosis, and the average survival rate of patients with current treatments is only 25-30 months.
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Science Advances: PARP1 inhibitors are expected to be an effective treatment strategy for PDAC
Time of Update: 2020-12-31
same time, the researchers also found that the MYBBP1A protein works on the nucleal DNA of cells to promote the activity of growth genes and interacts closely with proteins called PARP1 proteins.
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SUMO Fusion Technology for Enhanced Protein Production in Prokaryotic and Eukaryotic Expression Systems
Time of Update: 2020-12-27
Following the expression and purification of the fusion protein, the SUMO-tag can be cleaved by specific (SUMO) proteases via their endopeptidase activity in vitro to generate the desired N-terminus of the released protein partner.
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Zebrafish as a Model to Study Chemokine Function
Time of Update: 2020-12-14
Due to their optical clarity, they areuniquely suited for time-lapse imaging studies, providing insights into the dynamic processes underlying tissue formationand cell migration.
Wefurthermore illustrate how to image migrating cell populations using time-lapse microscopy in double-transgenic embryos.
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RNAi in Living Mice
Time of Update: 2020-11-23
It was demonstrated that the transgenically expressed, double stranded RNA could silence the GFP throughout the body of the mouse or rat and throughout the lifetime.
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Detection of hCG responsive expression of the steroidogenic acute regulatory protein in mouse leydig cells
Time of Update: 2020-11-22
Stimulation of mLTC-1 mouse Leydig tumor cells with hCG resulted in the coordinate regulation of StAR mRNA expression and progesterone accumulation in a time-response manner.
Collectively, the experimental approaches utilized in the present investigation allow for the demonstration and characterization of hCG mediated regulation of StAR mRNA and StAR protein expression in mouse Leydig cells.
