42% lower risk of death! The team of Ye Dingwei of Fudan University released the first Chinese plan for the treatment of metastatic hormone-sensitive prostate cancer
Time of Update: 2022-09-21
On September 5, 2022, the team of Ye Dingwei of the Department of Urology of the Affiliated Cancer Hospital of Fudan University published a research paper in the journal Lancet Oncology, which lasted more than 4 years, comparing the efficacy and safety of "revirutrum combined castration therapy" and the classic "bicalutamide combined castration therapy" in patients with high tumor load metastatic hormone-sensitive prostate cancer, confirming the first new second-generation anti-male drug "revirutamine" independently developed by China.
How Cell Metabolism Controls Appetite at the Source—Providing New Approaches to Diabetes and Obesity Treatment
Time of Update: 2022-06-08
Chopra from the Harrington Institute for Discovery in the United States published an article titled Protein tyrosine phosphatase receptor d serves as the orexigenic asprosin receptor in the journal Cell Metabolism, identifying the receptor that regulates the orexigenic activity of Asprosin— - protein tyrosine phosphatase receptor d (Ptprd) .
Why do the two most famous "artificial meat" in the world use different protein
Time of Update: 2021-06-28
Therefore, the selling point of IF is a greater degree of "simulation"-and soy protein can produce muscle fiber taste better than pea protein, so using it is also a good choice .
Factory production of new varieties of plants
Time of Update: 2021-03-13
If we use pre-cultured plant cell protosomes for sophyte fusion, we can also quickly obtain a large number of hybrid cells.
addition, pollen cell protosumin culture and chromosomal double technology provides excellent technical means for rapid purification of specific plant line.
Reconstitution of the SecY Translocon in Nanodiscs
Time of Update: 2021-01-05
Secretory proteins are transported across the bacterial envelope using a membrane protein complex called the SecY channel or translocon.
The technology is simple, yet should lead to additional new progresses in the field of membrane protein transport.
Purification and Functional Reconstitution of the Bacterial Protein Translocation Pore, the SecYEG Complex
Time of Update: 2020-12-18
The ability to study the activity of the translocase in vitro using purified proteins has been instrumental for our understanding of the mechanisms underlying this process.
Here, we describe the protocols for the purification and reconstitution of the SecYEG complex in an active state into liposomes.
Cell-Free Protein Synthesis for Analysis by NMR Spectroscopy
Time of Update: 2020-12-13
Cell-free protein synthesis offers fast and inexpensive access to selectively isotope labeled proteins thath can be measured byNMR spectroscopy in the presence of all the unlabeled proteins in the reaction mixture.
This chapter describes in detail the protocol used in the authors' laboratory.
Fractionated Extraction of Total Tissue Proteins from Mouse and Human for 2-D Electrophoresis
Time of Update: 2020-12-09
The protocol for extracting proteins from mouse and human tissues (organs) described in this chapter follows a strategy that is based on the intention to include all the various protein species of a particular tissue in a set of samples that are suitable for two-dimensional electrophoresis (2-DE), particularly for the large gel 2-DE technique described in Chapter 18 .
SPR Biosensor as a Tool for Screening Prion Protein Binders as Potential Antiprion Leads
Time of Update: 2020-12-06
No effective treatments are currently available for the diseases, vCJD in particular.
It is believed that the formation of protease-resistant insoluble prion protein (PrP Sc ), which is the main component of amyloidal deposits, from the cellular prion protein (PrP C ), is essential for the progression of the disease.
Site-Specific Labeling of Proteins for Single-Molecule FRET Measurements Using Genetically Encoded Ketone Functionalities
Time of Update: 2020-11-29
Proteins were purified with high yield and subsequently labeled with the hydroxylamine derivative of Alexa Fluor� 488 functioning as a fluorescent donor dye.
The proteins were then labeled via maleimide coupling chemistry at a unique cysteine with the acceptor dye Alexa Fluor� 594 to yield a dual-labeled protein ready for subsequent smFRET observation.
Single-Molecule Protein Unfolding and Refolding Using Atomic Force Microscopy
Time of Update: 2020-11-27
Over the past few years, atomic force microscopy (AFM) became a prominent tool to study the mechanical properties of proteins and protein interactions on a single-molecule level.
For example, AFM offered new insights into the process of protein folding and unfolding by probing single proteins with mechanical forces.
Mass Spectrometric Analysis of Mucin Core Proteins
Time of Update: 2020-11-24
Mucins are difficult to handle for their identification and characterization via proteomic applications due to their heavily glycosylated nature (up to 90%), high molecular weight (200 kDa–200 MDa), and size (Rg 10–300 nm).
Determining the Location of an Arabidopsis Chloroplast Protein Using In Vitro Import Followed by Fractionation and Alkaline Extraction
Time of Update: 2020-11-10
Determining the sub-organellar location of a protein in the chloroplast is vital for understanding or verifying the function of the protein.
Here, we present protocols for determining the sub-organellar location of a chloroplast protein.
Two-Dimensional Nanoflow Liquid Chromatography-Tandem Mass Spectrometry of Proteins Extracted from Rice Leaves and Roots
Time of Update: 2020-11-08
The protocol involves harvesting of leaves and roots from rice plants, preparing protein extracts from the harvested tissues, preparing proteolytic digests of the extracted proteins, making a biphasic capillary column with an integral electrospray emitter, performing two-dimensional chromatographic separation of peptides with data-dependent tandem mass spectrometry, and the use of database searching of the acquired tandem mass spectra to identify peptides and proteins.
Separation, Purification, and Characterization of Polypeptide Composition of Subcomplexes of the Main Light-Harvesting Chlorophyll a/b-Protein Complex of Photosystem II
Time of Update: 2020-11-07
The protocols regarding separation and purification of LHCII subcomplexes by non-denaturing isoelectric focusing (ndIEF), confirmation of trimeric nature of the subcomplexes, and establishing their p