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An In Vivo UV Crosslinking Assay That Detects DNA Binding by Sequence-Specific Transcription Factors
Time of Update: 2021-01-31
For many of these proteins, the pattern of DNA binding discovered by UV crosslinking differs dramatically from that predicted by earlier indirect approaches.
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Reconstitution and Analysis of Hyperacetylated Chromatin
Time of Update: 2021-01-31
Specific acetylation at conserved lysines in the N-terminal tails of histones have been correlated with distinct chromatin structures, association of specific chromatin proteins, accessibility of nucleosomalDNA toward interaction of transcription factors, and unfolded chromatin with increased transcription potential ( 1 – 5 ).
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Fluorescence and Brightfield Cytology of Live M. tuberculosis Cells
Time of Update: 2021-01-31
Although light microscopy fell out of favor as a research tool in prokaryotic biology in the 1980s, advances in the reagents available for cell labeling (staining) and in the user-friendliness of microscopes were underpinning a revolution in eukaryotic cell biology.
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32 Cases of Peptic Ulcer Treated by Dele Plus Erbailiwei Powder
Time of Update: 2021-01-31
Network Delega di-white gastric dispersal treatment of peptic ulcers 32 cases peptic ulcers refers to the stomach and 12-finger intestinal mucous membrane layer, mucous membrane lower layer or muscle layer ulcers, stomach small bends, claustrophobia near the common, men are more common in women clinical characteristics are chronic, rhythmic and periodic upper abdominal pain, and accompanied by symptoms such as gas, anti-acid.
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Preparation of Blotted Membrane for Protein Microsequencing
Time of Update: 2021-01-31
This chapter aims to provide a step-by-step procedure in preparing protein samples of limited quantities for partial sequence analysis.
Detailed descriptions will be focused on how to electroblot protein samples onto polyvinylidene fluoride (PVDF) membrane.
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Bacteriophage Typing
Time of Update: 2021-01-31
Bacteriophages (phages) are viruses that infect bacteria.
Susceptibility to infection by particular phages varies between strains within a species, and this property can be exploited to construct highly discriminatory schemes for the type identification of strains in epidemiological studies.
Part of the success of the technique is due to the simple methodology involved.
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Development of Improved Strains and Optimization of Fermentation Processes
Time of Update: 2021-01-31
Advances in recombinantDNA technology have made it possible for engineering improved microbial strains by specific addition or deletion of certain genes.
Finally, new technologies and how they can help strain development and fermentation process optimization will be discussed.
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32 Cases of Peptic Ulcer Treated by Dele Plus Erbailiwei Powder
Time of Update: 2021-01-31
Network Delega di-white gastric dispersal treatment of peptic ulcers 32 cases peptic ulcers refers to the stomach and 12-finger intestinal mucous membrane layer, mucous membrane lower layer or muscle layer ulcers, stomach small bends, claustrophobia near the common, men are more common in women clinical characteristics are chronic, rhythmic and periodic upper abdominal pain, and accompanied by symptoms such as gas, anti-acid.
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SMEAR PREPARATION
Time of Update: 2021-01-31
The preparation of a smear is required for many laboratory procedures, including the Gram-stain.
A smear can be prepared from a solid or broth medium.
If using a broth medium, do not add the water.
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Integrated Cell Culture/PCR for Detection of Enteric Viruses in Environmental Samples
Time of Update: 2021-01-31
Recently, an integrated cell culture/polymerase chain reaction (ICC/ PCR ) technique has been developed for the detection of viruses in environmental samples providing a reliable method for practical analysis and direct monitoring of environmental samples for viral pathogens ( 1 , 2 ).
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Methods for Detecting Antimeasles, Mumps, and Rubella Virus Antibodies
Time of Update: 2021-01-31
Rubella virus is a member of theTogaviridae family containing a positive-stranded RNA genome encoding both nonstructural and structural viral proteins (3 ).
(previous puge ) Schematic representation of measles, mumps, and rubella viruses, then genome structure, andSDS -PAGE analysis of viral structural protein.
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Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models
Time of Update: 2021-01-31
Histopathology is a defining endpoint in mouse models of experimental gastritis and gastric adenocarcinoma.
Presented here is an overview of the histology of gastritis and gastric cancer in mice experimentally infected withHelicobacter pylori orH.
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Genetic Manipulation of Xanthophyllomyces dendrorhous and Phaffia rhodozyma
Time of Update: 2021-01-30
Microbiological and genetic techniques for manipulation are rudimentary in the yeast, while their utility would be valuable for strain development including hypermutants that overproduce astaxanthin.
These methods are valuable for understanding the biology and enhancing the biotechnology value of the yeast.
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Listeria monocytogenes
Time of Update: 2021-01-30
Listeria monocytogenes was discovered as a pathogen of animals and humans in the 1930s.
monocytogenes in particular, are naturally occurring in a wide variety of domestic animals, particularly sheep and chickens.
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Electrotransformation in Salmonella
Time of Update: 2021-01-30
Enteric bacteria are not naturally competent forDNA -mediated transformation, but methods derived from the Ca 2+ -shock method of Mandel and Higa ( 1 ) permit uptake of DNA at levels adequate for molecular cloning.
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Differentiation of Fungi Using Hybridization Probes on the LightCycler
Time of Update: 2021-01-30
Today, hybridization probes are often used in real-timePCR for more sensitive and specific detection of pathogens and for determination of gene regulation or mutation analysis instead of intercalating dyes like SYBR Green.
Furthermore, we show the advantages of hybridization probes using the LightCycler-PCR for the detection of differentCandida spp.
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Analysis of Protein-Protein Interactions During Cytomegalovirus Infection
Time of Update: 2021-01-30
However, such interaction need not necessarily be direct, as immunoprecipitation of a viral protein that could interact with a single component of a multiprotein complex might be expected to co-precipitate all the proteins in that complex.
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Assessing Survival of Dairy Propionibacteria in Gastrointestinal Conditions and Adherence to Intestinal Epithelia
Time of Update: 2021-01-30
The genus Propionibacterium consists of two principal groups,cutaneous andclassical ordairy . Cutaneous species are predominant members of the microbial population of human skin and have also bee
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Yeast Systems Biology: The Challenge of Eukaryotic Complexity
Time of Update: 2021-01-30
In this chapter, we present an up-to-date view of the optimal characteristics of the yeastSaccharomyces cerevisiae as a model eukaryote for systems biology studies, with main molecular mechanisms, biological networks, and sub-cellular organization essentially conserved in all eukaryotes, derived from a complex common ancestor.
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Ultrasensitive Detection of Botulinum Neurotoxins and Anthrax Lethal Factor in Biological Samples by ALISSA
Time of Update: 2021-01-30
The detection method encompasses two steps: (1) specific target capture and enrichment and (2) cleavage of a fluorogenic substrate by the immobilized active target, the extent of which is quantitatively determined by differential fluorometry.
