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Human Intestinal Dendritic Cell Isolation
Time of Update: 2021-01-26
The isolation of dendritic (DC) cells from human intestinal tissue is associated with many of the problems encountered in the mouse, for example, low cell numbers and the paucity of specific cell surface markers.
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Isolation and Light-Stimulated Expression of Canthaxanthin and Spirilloxanthin Biosynthesis Genes from the Photosynthetic Bacterium Bradyrhizobium sp. Strain ORS278
Time of Update: 2021-01-26
strain ORS278, which synthesizes, in addition to the photosynthetic carotenoid spirilloxanthin, large amounts of canthaxanthin.
The procedure we developed to study the effect of the light on carotenoids synthesis is also described.
Finally, we describe a procedure to genetically transform photosyntheticBradyrhizobium strain ORS278 for improvement of canthaxanthin production.
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30 Cases of Peptic Ulcer Treated by Dele
Time of Update: 2021-01-26
2 results 29 cases of symptoms in the experimental group disappeared, and X-ray and gastroscopic review of the original ulcer healed with 95% efficacy.
In the control group, only 18 cases of symptoms disappeared, ulcers healed, and the effect was 60%.
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Respiratory Syncytial Virus and Other Pediatric Respiratory Virus Infections
Time of Update: 2021-01-26
Of these the most commonly diagnosed in the pediatric population is respiratory syncytial virus (RSV), which infects essentially all children in their first or second year of life, bringing approx 1% into the hospital with bronchiolitis or pneumonia ( 1 ).
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Optimized Multiplex Polymerase Chain Reaction: An Effective Method for Rapid Gap Closure
Time of Update: 2021-01-26
Optimized Multiplex PCR ( 2 ) provides an effective new method for the rapid closure of a large number of gaps.
Unlike combinatorial PCR, Optimized Multiplex PCR minimizes the number of reactions by combining primers into pools and testing these pools against each other.
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Proteome Analysis of Adenovirus Using Mass Spectrometry
Time of Update: 2021-01-26
For analysis of viral proteomes, an optimal protocol includes production of a highly purified virus that can be investigated with a high-resolving analytical method.
This method provides information on the chemical composition of the virus particle.
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PCR for the Detection of H. pylori in Gastric Juice Aspirates and Environmental Water Samples
Time of Update: 2021-01-26
Helicobacter pylori is a curved Gram-negative bacillus that infects the human gastric mucosa.
pylori require the collection of gastric samples by biopsy during gastroduodenoscopy.
To make a diagnosis without gastroduodenoscopy, indirect tests, such as ELISA or urea breath tests, must be relied on ( 2 ).
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Determination of rRNA Gene Restriction Patterns
Time of Update: 2021-01-26
The development of molecular biology has opened the way to new approaches to bacterial identification and typing.
The number and position of these endonuclease-specific restriction sites on a DNA molecule determine the number and the sizes of the fragments generated by cleavage.
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Import of Precursor Proteins Into Isolated Yeast Mitochondria
Time of Update: 2021-01-26
In addition to their metabolic role, mitochondria are also key players in cellular apoptosis and participate in the generation of reactive oxygen species (ROS) and in calcium signaling.
The process of mitochondrial protein import can be reconstituted in vitro using isolated mitochondria and in vitro synthesized precursor proteins.
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Peptic ulcer gastric fluid sIgA with Helicobacter pyridobacteria infection
Time of Update: 2021-01-26
Network Peptic ulcer gastric fluid sIgA and Helicobacter pyridosis infection 1 object and method 1.1 object all cases were examined and pathologically confirmed by the gastroscopy room of our hospital, of which 31 cases of gastric ulcer (GU), an average of 39 years old; In 30 cases in the control group, with an average age of 32.7 years, gastroscopic biopsies confirmed that there were no significant abnormalities.
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Chromatographic Analysis of the Escherichia coli Polysialic Acid Capsule
Time of Update: 2021-01-26
Polysialic acid capsules are the major virulence factors inEscherichia coli K1, K92, and groups B and C meningococci.
Here, we describe methods to release the constituent capsular polysialic acid monomers, and to detect and quantify them by sensitive fluorometry.
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Genome Plasticity: Insertion Sequence Elements, Transposons and Integrons, and DNA Rearrangement
Time of Update: 2021-01-26
This chapter discusses bacterial DNA transfer systems, transposable elements and integrons, and the contributions each makes towards the evolution of bacterial genomes, particularly in relation to bacterial pathogenesis.
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Enzyme-linked immunology analysis of S2 antibodies (HBV preS2Ab) before human hepatitis B virus
Time of Update: 2021-01-26
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Enzyme-linked immunology analysis (ELISA) of S1 antibodies (HBV preS1Ab) before human hepatitis B virus
Time of Update: 2021-01-25
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Capture ELISA Quantitation of Mouse Adenovirus Type 1 in Infected Organs
Time of Update: 2021-01-25
A capture enzyme-linked immunosorbent assay (ELISA) was optimized for identification of mouse adenovirus type 1 in infected brain homogenates.
Methods for organ homogenization and the subsequent capture ELISA are described in this chapter.
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Applications for Green Fluorescent Protein in Cell Signaling
Time of Update: 2021-01-25
Expression vectors are now supplied commercially that allow proteins to be tagged easily ( 6 , 7 ).
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Genome-Wide Measurement of Histone H3 Replacement Dynamics in Yeast
Time of Update: 2021-01-25
Chromatin plays critical roles in processes governed in different timescales – responses to environmental changes require rapid plasticity, while long-term stability through multiple cell generations requires epigenetically heritable chromatin.
Understanding the dynamic behavior of chromatin is of great interest for fields ranging from transcriptional regulation through meiosis and gametogenesis.
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Gene Fusions for the Directed Modification of the Carotenoid Biosynthesis Pathway in Mucor circinelloides
Time of Update: 2021-01-25
In this chapter we describe part of our efforts to construct active gene fusions which could advance in the diversification of carotenoid production by this fungus.
to thecrtS gene ofXanthophyllomyces dendrorhous , responsible for the synthesis of astaxanthin from β-carotene in this fungus, but which was shown to have only hydroxylase activity inM.
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Using Reporter Genes and the Escherichia coli ASKA Overexpression Library in Screens for Regulators of the Gram Negative Envelope Stress Response
Time of Update: 2021-01-25
coli ASKA overexpression library for clones that lead to altered expression of reporter genes.
The plasmids in each well are transformed into the reporter strain and transformants are screened for either altered β-galactosidase or light production.
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Enzyme-linked immunology analysis of piglet microvirus antibodies (RPV).
Time of Update: 2021-01-25
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