PCR for the Detection of H. pylori in Gastric Juice Aspirates and Environmental Water Samples
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Last Update: 2021-01-26
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Source: Internet
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Author: User
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Helicobacter pylori
is a curved Gram-negative bacillus that infects the human gastric mucosa. Most methods used to diagnose
H. pylori
require the collection of gastric samples by biopsy during gastroduodenoscopy. A positive culture or visualization of the organism by histologic examination of biopsy material can give a definitive diagnosis (
1
). To make a diagnosis without gastroduodenoscopy, indirect tests, such as ELISA or urea breath tests, must be relied on (
2
). The polymerase chain reaction (
PCR
) can be used to make a highly specific diagnosis from gastric juice aspirates or environmental samples.
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