 Home > Biochemistry News > Microbiology News > Chromatographic Analysis of the Escherichia coli Polysialic Acid Capsule

Chromatographic Analysis of the Escherichia coli Polysialic Acid Capsule

 Last Update: 2021-01-26
 Source: Internet
 Author: User

Tags

what escherichia coli

1 4 liter

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

Polysialic acid capsules are the major virulence factors in
Escherichia coli
K1, K92, and groups B and C meningococci. The sialic acid monomers (2-keto-3-deoxy-5-acetamido-7,8,9-
d-
glycero
-
d-
galacto
-nonulosonic acids) comprising these homopolymeric polysaccharide chains can be selectively modified with 1,2-diamino-4,5-methylenedioxy-benzene to produce highly fluorescent quinoxalinone derivatives distinguished by their elution times during reverse phase chromatography. Here, we describe methods to release the constituent capsular polysialic acid monomers, and to detect and quantify them by sensitive fluorometry. There are relatively few 2-keto acids in bacteria, making it possible to rapidly analyze samples even without prior purification of capsular polysaccharides.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

African swine fever B646L plasmid quality control BNCC359820 BNCC

CAS No.:

$220-280/MT EXW

Standard quality control sample of fecal coliform (filter membrane method) BNCC359578 BNCC

CAS No.:

$220-280/MT EXW

Reference substance for total number of colonies in food BNCC359091 BNCC

CAS No.:

$220-280/MT EXW

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

announcements today(81)

80 20 company(94)

united states logo(227)

single use technology(195)

healthy food name(42)

ups united states(221)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.