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Differentiation of Fungi Using Hybridization Probes on the LightCycler

 Last Update: 2021-01-30
 Source: Internet
 Author: User

Tags

molecular chain

domino chain reaction

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The polymerase chain reaction is a powerful molecular tool for the detection and analysis of very small amounts of
DNA
. Today, hybridization probes are often used in real-time
PCR
for more sensitive and specific detection of pathogens and for determination of gene regulation or mutation analysis instead of intercalating dyes like SYBR Green. Here, we describe how to generate suitable primers and hybridization probes for the specific detection of fungal DNA. Furthermore, we show the advantages of hybridization probes using the LightCycler-PCR for the detection of different
Candida
spp. and
Aspergillus
spp. in patient blood samples. The methods used to develop such PCR assays will also be presented in the following protocol.

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