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Assay Methods for Detection and Quantification of Antimicrobial Metabolites Produced by Streptomyces clavuligerus
Time of Update: 2021-01-27
Streptomyces clavuligerus is used as model to illustrate the determination and quantification of antimicrobial metabolites with different biological activities produced by cultures of this strain.
S. clavuligerus produces an array of compounds with different structures.
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Single-Strand Conformation Polymorphism Analysis for the Diagnosis of T-Cell Clonality in Periodontal Disease
Time of Update: 2021-01-27
T-cell clonal expansion has been detected in the peripheral blood or the disease-affected sites in patients with infections, autoimmune diseases, malignancy, and post-transplantation complications.
Since antigen stimulation of T cells induces the proliferation of specific T cells, clonal T-cell expansion is considered to be a result of an antigen-specific immune response.
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Production of Erythromycin With Saccharopolyspora erythraea
Time of Update: 2021-01-27
Actinomycetes are among the most fascinating microorganisms.
Their developmental life cycle with its morphological and physiological differentiation and the rich repertoire of secondary metabolites (about 70-80% of bioactive secondary metabolites are being produced by actinomycetes) have resulted in a large research community studying these microbes.
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Nucleotide Excision Repair Coupled to Chromatin Assembly
Time of Update: 2021-01-27
A step toward the dissection of these events was recently accomplished by the development of an assay for both chromatin assembly and NER on the same DNA molecules in cell-free systems competent for the two processes ( 2 , 3 ).
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Affinity Isolation of Antigen-Specific Circulating B Cells for Generation of Phage Display-Derived Human Monoclonal Antibodies
Time of Update: 2021-01-27
This approach should overcome the problem of low yields of monoclonal antibodies of interest in the libraries generated from peripheral blood lymphocytes caused by the low abundance of antigen-specific B cells in the circulation.
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Generation of Envelope-Modified Baculoviruses for Gene Delivery Into Mammalian Cells
Time of Update: 2021-01-27
Genetically modified baculoviruses can efficiently deliver and express genes in mammalian cells.
Methods of generating a recombinant baculovirus vector with a modified envelope and its amplification and purification, including technical scale production, are discussed.
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Chlamydia psittaci
Time of Update: 2021-01-27
Parrot chlamydia is easy to grow in chicken embryo yolk sacs and HeLa cells, monkey renal cells culture and can infect mice with pneumonia, periaryitis or encephalitis and die.
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Network Analysis and Protein Function Prediction with the PRODISTIN Web Site
Time of Update: 2021-01-27
Interactions between macromolecules are deciphered to gain information about biological processes and protein function.
In this context, the PRODISTIN Web site is dedicated to the clustering of network proteins according to the identity of their interaction partners, and to the subsequent functional annotation of these clusters.
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ET Recombination: DNA Engineering Using Homologous Recombination in E. coli
Time of Update: 2021-01-27
Recombinogenic engineering, or the modification and cloning of DNA molecules via homologous recombination, has opened up a new era.
Because homologous recombination is a precise process of high fidelity, recombinogenic engineering generates DNA clones efficiently and with high precision.
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Preparation of Soluble Extracts From Adenovirus-Infected Cells for Studies of RNA Splicing
Time of Update: 2021-01-27
Here we describe a collection of methods that have been adapted to produce highly efficient nuclear and cytoplasmic extracts from adenovirus-infected HeLa cells. We describe how to produce extracts f
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Fluorescent Reporters and Methods to Analyze Fluorescent Signals
Time of Update: 2021-01-27
The use of fluorescent reporters and the development of new imaging technologies have revolutionized studies in cell biology.
This chapter presents an overview of fluorescent reporters and methods available, and describes a selection of those that are routinely applicable in imaging studies usingDictyostelium discoideum .
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Introduction: Medical Significance of H. pylori
Time of Update: 2021-01-27
Until the discovery ofHelicobacter pylori in 1982, the normal human stomach was generally considered to be sterile, or transiently populated by oropharyngeal bacteria carried there by peristalsis.
However, we now know that from one-third to one-half of the human population carriesH.
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Genetic Transformation of Bacteroides spp. Using Electroporation
Time of Update: 2021-01-27
Thus, much of the work onBacteroides genetics focused on analysis of transmissible antibiotic resistance determinants, and these resistance elements have in turn provided many of the plasmids and selective markers used in the development ofBacteroides genetics ( 1 – 3 ).
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Luminescence as a Continuous Real-Time Reporter of Promoter Activity in Yeast Undergoing Respiratory Oscillations or Cell Division Rhythms
Time of Update: 2021-01-27
This chapter describes a method for generating yeast respiratory oscillations in continuous culture and monitoring rhythmic promoter activity of the culture by automated real-time recording of luminescence.
Additionally, this chapter describes a method for observing rhythmic (cell cycle-related) promoter activity in small batch cultures of yeast through luminescence monitoring.
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The head of the 鲂
Time of Update: 2021-01-27
Spawning period in 5 to 6 months, spawning sites generally need to have a certain amount of running water, there is dense water Grass, the bottom is soft mud and sand, water depth 1.0 to 1.5 meters, water Temperature 20 28 degrees Celsius, 2 The amount of eggs in winter is 3.7 to 10.3 br.3 br / > 10,000 grains.
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Fast Atom Bombardment-Mass Spectrometry
Time of Update: 2021-01-27
Mass spectrometry (MS) is a chemical analytical technique that can yield data in the form of a spectrum of peaks of differing relative intensities and over a range of mass-to-charge (m/z) values.
Recently, a new method for ionization of analyte has been devised, fast atom bombardment (FAB).
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A Non-Human Primate Model of Acute Group A Streptococcus Pharyngitis
Time of Update: 2021-01-27
Humana Press, Totowa, NJ This chapter describes methods for using non-human primates as a model of group A streptococcal (GAS) pharyngitis.
This model has been used successfully to study host–pathogen interactions occurring during pharyngeal GAS infections.
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Molecular Phylogenetics: Testing Evolutionary Hypotheses
Time of Update: 2021-01-27
This methodology is known as molecular phylogenetics and may be the most informative means for exploring phage evolution, since there are few morphological features that can be used to differentiate between these tiny biological entities.
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Lyophilizing Cells
Time of Update: 2021-01-27
Resuspend each pellet in 10 ml (1/10 volume) sterile 1x PBS, and recombine into a 50-ml conical bottom, polypropylene centrifuge tube.
Place the frozen tube, with the cap loosened, in the glass lyophilization vessel.
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Mycoplasma Gene Expression in Escherichia coli
Time of Update: 2021-01-27
Using hyperimmune antisera, it has been possible to identify some cloned genes because of the probability of having antibodies directed against amino-terminal regions of the truncated polypeptides, and obviously, gene sequences lacking UGA codons are easily identified.
