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Real-time quantification, are you really doing it right?
Time of Update: 2021-08-09
The RACE test before real-time quantitative PCR is mainly for ① to determine the full-length sequence of the functional gene in the experimental sample; ② more critically, to determine whether the target gene has alternative splicing (also known as alternative splicing), variable The number of splicing and the corresponding sequence .
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The French company Stilla Technologies released the development project of the high-throughput digital PCR system Naica HT and the 6-color digital detection system
Time of Update: 2021-08-09
Caroline Charky, Vice President of Commercial Operations of Stilla Company, released the high-throughput digital PCR system Naica HT and the new Opal chip at the SLAS Conference (Laboratory Automation and Screening Conference) in February 2019, which can detect ≥240 samples per day.
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Handheld ultra-fast real-time fluorescent quantitative PCR instrument assists on-site detection
Time of Update: 2021-08-09
Recently, Primetech (Beijing) Laboratory Technology Co. , Ltd. and Ahram Biosystems jointly demonstrated a mobile multiple nucleic acid detection system in Beijing . The core of the system is Ahram
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New digital PCR platform for three-channel detection [New product recommendation]
Time of Update: 2021-08-09
"We are extremely satisfied with the performance of the Naica™ crystal droplet digital PCR system, which can fully meet our requirements for precision and repeatability, and the operation is also extremely convenient, the software is intuitive, and the data analysis is fast .
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Template Preparation in Digital PCR——Gene-π Class 3
Time of Update: 2021-08-09
Therefore, template preparation is a key step for the success of digital PCR experiments .
Quality ControlIn order to obtain the best digital PCR performance, the purity and quality of the template should be checked .
Other parameters inherent in the sample itself may affect the digital PCR experiment:1.
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Facial recognition, voice commands: qPCR system is also intelligent
Time of Update: 2021-08-09
Thermo Fisher Scientific recently launched its first intelligent qPCR instrument-QuantStudio 6 & 7 Pro real-time quantitative PCR system .
These two instruments have multiple intelligent functions, including facial recognition, voice commands, RFID flatbed scanning, and smart help, etc.
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Scientists focus on the role of ddPCR technology in blood disease monitoring
Time of Update: 2021-08-09
RT-qPCRIn monitoring whether patients with chronic myeloid leukemia (CML) respond to tyrosine kinase inhibitors, detecting the level of the BCR-ABL1 fusion transcript is an effective method .
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A new choice for high versatility and high fidelity PCR enzymes of Premix type!
Time of Update: 2021-08-09
This product is a high-fidelity PCR enzyme PrimeSTAR GXL DNA Polymerase (Code No. R050) with both high accuracy and extension performance, adding the components required for PCR (such as buffer, dNTP, etc.
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Principle and Application of Digital PCR-One of the Gene-π Classes
Time of Update: 2021-08-09
The number of starting molecules, although the concept of "digital PCR" is not clearly put forward, the basic experimental procedure of digital PCR has been established, and an extremely important principle in digital PCR detection has been determined-"the presence or absence of end-point signal" As a quantitative method .
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High-throughput, nanoliter system qPCR system-SmartChip Real-Time PCR System
Time of Update: 2021-08-09
SmartChip Real-Time PCR System is a complete and flexible high-throughput qPCR solution, bringing you a different qPCR research experience .
SmartChip chip Rich combination of Assay and Sample SmartChip Real-Time PCR SystemResponsive and efficientThe SmartChip system uses a 100 nL reaction system, which significantly saves reagent costs .
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Naica high-throughput droplet chip-based digital PCR system
Time of Update: 2021-08-09
The Naica high-throughput droplet chip digital PCR system is a technical tool to achieve absolute quantification of DNA/RNA. It automatically generates and assembles single-layer flat droplets, perfo
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Human cancer EGFR gene multiplex digital PCR screening kit
Time of Update: 2021-08-09
Apexbio Human Cancer EGFR Gene Digital PCR Screening Kit, based on digital PCR platform and fluorescent probe method, is used for rapid and sensitive multiple screening of human cancer EGFR gene mutation sites (mutation) .
Internal control and quality control are set in the kit, which increases the validity and reliability of the data .
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Extremely fast real-time fluorescent quantitative PCR instrument running in your palm
Time of Update: 2021-08-09
Palm™ PCR S1, as an advanced portable instrument, helps you complete real-time PCR detection anytime and anywhere, without being restricted by the environment, power, and communication .
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Azure Cielo™ Real-time PCR System
Time of Update: 2021-08-08
The Azure Cielo™ real-time fluorescent quantitative PCR system launched by Azure Biosystems of the United States in 2020 combines high-quality Peltier temperature modules and optical detection systems based on optical fiber transmission to provide high-precision, high-sensitivity and reliable results for your scientific research .
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Naica™ 6-channel microdrop digital PCR system
Time of Update: 2021-08-08
Naica™ 6-channel droplet digital PCR system is a technical tool to achieve absolute quantification of DNA/RNA.
, To identify the effective negative and positive droplets in the six-color fluorescence, so as to achieve the absolute quantitative detection of the target DNA/RNA .
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Traveling far and wide, working hard—Introduction to the basics of PCR (1)
Time of Update: 2021-08-08
PCR reactions appropriate choice of gradient PCR, PCR was carried out according to the conditions set sizes of DNA fragments and an enzyme preparation instructions recommended .
Enzyme, buffer (Mg 2+ ), dNTPAs mentioned before, commercial DNA polymerases generally contain an appropriate concentration of dNTP and the most suitable reaction buffer.
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Scientists prove the value of ddPCR in quantitative cancer markers
Time of Update: 2021-08-08
Nell, a researcher at Leiden University Medical Center in the Netherlands, has developed a new method that can absolutely quantify the amount, density, and allele specificity of DNA methylation .
Nell combined methylation-sensitive restriction enzymes with ddPCR technology to develop a bisulfite-independent method to quantify epigenetic markers .
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Stilla Technologies launches a new reagent product line--naica
Time of Update: 2021-08-08
Stilla Technologies launched a new reagent product line-naica ® dPCR Mixes in November 2020 for digital PCR analysis . In order to improve detection efficiency and higher detection sensitivity, a PC
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Swine influenza virus RT-PCR detection method
Time of Update: 2021-08-08
Today, I will introduce a PCR technology-based swine flu virus detection method and Takara's corresponding star product, which is especially suitable for customers who have a large sample size and have certain requirements for detection accuracy and cost .
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[New Product Launch] QIAcuity Digital PCR System Launch Conference
Time of Update: 2021-08-08
QIAGEN's launch of the QIAcuity series of digital PCR systems in July 2020 has further advanced this revolution to a whole new level and defined a new era of digital PCR .
