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Multiplex PCR reagent with fast reaction speed & superior performance!
Time of Update: 2021-08-09
Among the several products compared, the cycle time of 20-fold PCR using SuperPlex Premix is the shortest, which saves a lot of time compared with competing products (see the table below for details) and the superior reaction performance as shown in the first experiment .
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Oncologists talk about the clinical application of ddPCR technology
Time of Update: 2021-08-09
Researchers used ddPCR-based liquid biopsy analysis to track ESR1 mutations associated with endocrine therapy resistance .
Although this research is still in progress, preliminary results show that ddPCR technology can quickly detect the presence of ESR1 mutations .
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The latest generation of digital PCR (cdPCR) landed in China [New product recommendation]
Time of Update: 2021-08-09
Welcome to request detailed information of Naica Crystal Digital PCR systemAbout Cycloud Deep Blue Cloud Deep Blue Cloud Biotechnology (Cycloudbio) is committed to providing you with new life science research instruments and analytical products as well as optimized overall application solutions.
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Human Cancer Her2 Gene Multiple Digital PCR Screening Kit
Time of Update: 2021-08-09
This kit is suitable for absolute quantitative analysis of DNA samples from peripheral blood (plasma), fresh tumor tissues, and paraffin-embedded sections of cells .
Internal control and quality control are set in the kit, which increases the validity and reliability of the data .
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Unbiased pre-amplification reagents to facilitate the research of low-initiated samples!
Time of Update: 2021-08-09
cDNA sample for reactionIn summary, Prelude™ PreAmp Master Mix can perform target-specific pre-amplification of limited or low-quality samples for downstream qPCR, SNP genotyping or TE NGS .
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Real-time quantification, are you really doing it right?
Time of Update: 2021-08-09
In addition to short transcripts that are related to human immune function, long transcripts and four moderate-length transcripts expressed in coronary artery endothelial or vascular smooth muscle cells are all involved in CAD .
First, researchers should use RACE technology or three-generation full-length transcriptome sequencing technology to determine whether the target gene has alternative splicing .
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Skip RNA purification and launch the new Cells-to-CT kit [New product recommendation]
Time of Update: 2021-08-09
The new Fast Advanced Cells-to-CT kit uses real-time fluorescent quantitative RT-PCR to directly detect relative gene expression in cultured cell samples, allowing you to skip tedious and time-consuming RNA purification steps .
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Will digital PCR change the monitoring and treatment of leukemia?
Time of Update: 2021-08-09
According to Patel, since the introduction of digital PCR in Bio-Rad in 2011, multiple research teams have developed alternative methods for measuring the level of BCR-ABL transcripts using ddPCR .
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Document Express Naica Digital PCR Detection of Cytokines
Time of Update: 2021-08-09
Recently, the Prion Laboratory of the Institute of Viral Disease Control and Prevention of the Chinese Center for Disease Control and Prevention published "IP10, KC and M-CSF is Remarkably Increased in the Brains from the Various Strains of Experimental Mice Infected with Different Scrapie Agents" in Virologica Sinica .
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Experimental design Drop-off digital PCR method two: gene mutation and gene deletion calculation method
Time of Update: 2021-08-09
Use Crystal Miner analysis software to quantitatively analyze Drop-off results step by stepThe wild-type concentration Cwt (double positive droplets) can be calculated directly by the ratio of Pwt (regardless of whether the third channel is positive or negative), and the concentration of drop-off mutation Cmut (mutant allele) can be calculated by Pmut .
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How to choose consumables for PCR experiments?
Time of Update: 2021-08-09
PCR single tubeAdopting unique one-shot injection molding technology, ultra-thin design, fast heat conduction, window lower than flat surface, not easy to be scratched, better sealing of the tube, almost zero evaporation, reducing aerosol pollution, and avoiding false positives to the greatest extent Produced .
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New product release QIAcuity integrated nanochip digital PCR system
Time of Update: 2021-08-09
QIAGEN's new digital PCR system QIAcuity based on integrated nanochips simplifies the experimental process to be as simple and fast as qPCR experiments .
The instrument automatically presses the sample into the nanopores of the microfluidic chip and seals each hole independently .
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Small and economical mini PCR machine, you deserve it
Time of Update: 2021-08-09
Many researchers need to carry out PCR experiments in the field, and a standard PCR machine (even a small desktop computer) obviously cannot meet their needs .
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Chinese scientists developed an improved version of multiple displacement amplification method [Innovative Skills]
Time of Update: 2021-08-09
The combined use of MDA and next-generation sequencing (NGS) has become the gold standard method for genome research .
The authors concluded that they developed a simple and reliable solution to eliminate template-independent amplification in MDA .
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Clontech new product awards help SNP genotyping
Time of Update: 2021-08-09
Among them, the restriction enzyme method based on electrophoresis detection (PCR-RFLP) is one of the more classic methods, but the premise of the application of this technology is that the site of the SNP must contain the recognition of the restriction enzyme.
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"0" PCR, the nemesis of the wrong InDel is here!
Time of Update: 2021-08-09
In the library construction stage of high-throughput sequencing, the library template is amplified thousands of times by PCR to achieve the library volume on the computer to complete the subsequent determination of the genomic DNA sequence .
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Direct amplification PCR-tissue/blood/plant sample preparation in 5 minutes
Time of Update: 2021-08-09
Loading dyes are included, which can be directly used for subsequent gel electrophoresis;In addition, the NucleoType Mouse PCR Direct Amplification Kit also provides a more time-saving direct PCR process, that is, the mouse tissue sample does not need to be lysed at room temperature, and the PCR reaction is directly performed .
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Black Hole Quencher® (BHQ) black hole quencher combined with full-band fluorescent dyes,
Time of Update: 2021-08-09
Here is a perfect solution with BHQ!In fact, Biosearch's fluorescent dyes are far more than these, and they have truly achieved full-band coverage!qPCR and multiple qPCR primer and probe design utility In addition to high-efficiency fluorescent quenching dyes and various >https:// ) With the fluorophore spectrum selection tool ( http://qpcrdesign.
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Naica™ crystal automatic microdrop chip digital PCR system
Time of Update: 2021-08-09
The Naica™ crystal automatic microdrop chip digital PCR instrument system is the next generation of absolute nucleic acid quantitative technology developed by the French company Stilla Technologies .
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How to determine the blank detection limit LOB of digital PCR?
Time of Update: 2021-08-09
LOB (Limit of Blank) is an important parameter to judge the detection ability of digital PCR technology .
When quantifying nucleic acids, the cutoff of false positives is critical to the stability of dPCR detection .
