Unbiased pre-amplification reagents to facilitate the research of low-initiated samples!

Prelude™ PreAmp Master Mix (Code No.

Pre-amplification experiments can help increase the initial amount of cDNA or gDNA for specific downstream target sequences.

Application-pre-amplification experiment

The 96-target cell cycle PrimerArray was used to quantify 100 pg cDNA without pre-amplification and 100 pg cDNA after 10 cycles of pre-amplification

Challenges and needs of pre-amplification experiments

When the PCR amplification efficiency of certain target sequences is low, deviations may occur, which may result in insufficient or excessive expression of the target sequence in the pre-amplified sample

▪ Outliers of pre-amplification may get false positives or false negatives

Cost is also a major factor; a single pre-amplification reaction is usually 10-25 times more expensive than standard PCR/qPCR reactions

need:

▪ Unbiased pre-amplification, it is best to amplify as many target sites as possible

How to measure the deviation of pre-amplification experiments?

▪ Comparative experiment between pre-amplified low-initiated cDNA and high-initiated cDNA without pre-amplification

How to measure the deviation of the pre-amplification experiment: Through the ΔCt value obtained in two experiments, calculate the ΔΔCt value and analyze it

Higher sensitivity

Compared with competing companies, cDNA samples have less deviation

Compared with competing companies, FFPE sample deviation is smaller

Use the 162-target breast cancer panel to detect FFPE samples

▪ Use NucleoSpin to extract total RNA in FFPE, and then use PrimeScript RT Master Mix for reverse transcription reaction, followed by 14 cycles of pre-amplification.

In summary, Prelude™ PreAmp Master Mix can perform target-specific pre-amplification of limited or low-quality samples for downstream qPCR, SNP genotyping or TE NGS

▪ High sensitivity, minimum 10 pg starting sample

Product information

For more details, please click:

Prelude™ PreAmp Master Mix