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Two unique new products of quantitative PCR instrument [New product recommendation]
Time of Update: 2021-08-10
In September last year, Bibby Scientific acquired PCRmax, and then launched the Eco 48 quantitative PCR instrument .
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PrimePCR™ Assays and Panels keep you away from the trouble of designing primers and probes
Time of Update: 2021-08-10
For PrimePCR™ Assays, each pair of primers for human, mouse, and rat genomes has been verified by qPCR experiments, and the verification report can be downloaded online, which complies with MIQE international guidelines to ensure data accuracy .
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Digital PCR to find diagnostic markers of breast cancer [Innovative Tips]
Time of Update: 2021-08-10
In this study, researchers at the University of Ferrara in Italy used Bio-Rad's QX200 droplet digital PCR (ddPCR) system to determine the absolute levels of key miRNA molecules in plasma and serum samples from a variety of cancer patients .
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It is said to be the smallest PCR detector in the world [new product recommendation]
Time of Update: 2021-08-10
Spartan Cube uses polymerase chain reaction (PCR) technology to carry out different DNA tests in as little as 30 minutes .
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The latest masterpiece of Applied Biosystems, ProFlex is on the market!
Time of Update: 2021-08-10
>• Use the existing experimental program-with thermal simulation mode, no need to change the PCR program, to achieve seamless transition of the instrument • Convenient remote connection-support Wi-Fi, use free mobile
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Plant Direct PCR Technology
Time of Update: 2021-08-10
>. >The original purpose of nucleic acid purification is to remove impurities that may interfere with subsequent reactions. For PCR technology, if you can skip this step and directly
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PCR alternative technology, common problems of RPA primers and probes
Time of Update: 2021-08-10
Do RPA primers need special purification?When performing primer screening, purification is generally not required .
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PCR machine, did you really choose the right one?
Time of Update: 2021-08-10
The aforementioned thermal overshoot of the module requires very rich experience, and an accurate algorithm can make samples of different volumes reach a stable set point temperature without overshoot and related negative effects .
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Detection of host cell residual DNA, ddPCR has many benefits [new product recommendation]
Time of Update: 2021-08-10
Compared with real-time quantitative PCR technology, the advantage of using droplet digital PCR (ddPCR) technology and premix is that the DNA purification step can be skipped and the residual DNA of the host cell can be directly determined .
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PCR alternative technology, primer design of RPA strategy
Time of Update: 2021-08-10
For the 3 ' end of the 3 nucleotides, the guanine and cytosine help stabilize polymerase binding, can improve the performance of the amplification primers .
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RPA technology test water library preparation for next-generation sequencing
Time of Update: 2021-08-10
>. PCR amplification is a standard library preparation procedure, but PCR</span
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Smaller, faster and stronger?
Time of Update: 2021-08-10
and cooling process of the heating module . > >Punch-it nucleic acid sample preparation Palm PCR machine><span
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Applied Biosystems' new heavy product-Automated PCR Instrument (ATC) [New Product Recommendation]
Time of Update: 2021-08-10
Applied Biosystems™ automated PCR instrument (ATC) design: • Composition: heating module and independent control module • Docking with automatic platform systems such as Tecan, Hamilton, etc.
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Instead of PCR, RPA plays an important role in HIV testing
Time of Update: 2021-08-10
A study in September of this year showed that when the room temperature is between 31°C-43°C , the RPA reaction can be successfully incubated for 20 minutes , and 10 copies of HIV-1 proviral DNA can be detected .
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How to distinguish the copy number variation of heterogeneous samples [Innovative Tips]
Time of Update: 2021-08-10
They recently released an application guide that combines cell sorting and digital PCR to distinguish copy number variations in heterogeneous samples .
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Instead of PCR, RPA has become a powerful tool for pathogen detection
Time of Update: 2021-08-10
In a series of articles published this year, RPA technology is widely used in the detection of common pathogenic bacteria such as Mycobacterium tuberculosis, drug-resistant bacteria MRSA , and Salmonella .
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A KRAS mutation detection method superior to sequencing [Innovative Tips]
Time of Update: 2021-08-10
Research, claiming that the peptide nucleic acid clamp PCR (PNA-PCR) technology is more sensitive than Sanger sequencing in detecting KRAS mutations . ><span
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Inhibitor-tolerant qPCR reagents are freshly launched [new product recommendation]
Time of Update: 2021-08-10
BioTone reported that Bio-Rad recently announced the launch of a PCR inhibitor-resistant qPCR master mix-SsoAdvanced™ Universal Inhibitor-Tolerant SYBR® Green Supermix, which can obtain high-quality qPCR data from the most challenging samples .
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Regarding digital PCR, please keep these dry goods [experience comment]
Time of Update: 2021-08-10
For Bio-Rad's droplet digital PCR system (ddPCR), approximately 1-100,000 copies of DNA target molecules are required per well .
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A new generation of SuperScript reverse transcriptase is on the market [new product recommendation]
Time of Update: 2021-08-10
According to Life Technologies, SuperScript® IV reverse transcriptase significantly improves resistance to a variety of inhibitors and can perform reliable and specific cDNA synthesis for a variety of sample types .
