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Applied Biosystems is a pioneer and leader in real-time quantitation technology and brand, and has created an excellent and complete real-time quantitative PCR solution platform in nearly 30 years of development and innovation - multi-model, multi-throughput real-time PCR system, full-featured software applications, comprehensive professional technical support and services, as well as a rigorous and complete library of primer probes and high-performance master mixes
for various applications.
In a wide variety of assays, finding a master mix that performs well, performs consistently, and has a reliable supply for a specific application is critical
in the real-time quantitation process.
In order to meet the needs of Chinese customers and meet the expectations of Chinese customers, Thermo Scientific invested in product design and R&D for two years, and concentrated on creating the Qiankun ™ Platinum ™ series qPCR master mix, which is now grandly listed
.
New Product 1: Applied Biosystems TaqMan Platinum One-Step Multimix (Available in October 2022)
Designed for rapid, multiplexed, and highly sensitive real-time real-time RT-PCR detection, high-quality results
even in complex samples.
Contains UDGase, which is resistant to contamination
.
10X enzyme master mix and 5X buffer to add more sample to each reaction, increasing sensitivity
even in small system reactions.
The separate format of the enzyme and buffer facilitates the optimization of the reaction system and is ideal for
molecular diagnostic applications.
New Product 2: Applied Biosystems TaqMan Platinum Multi Master Mix (Available in October 2022)
Designed for rapid, multiplexed, and highly sensitive real-time PCR detection, high-quality results
even in complex samples.
Contains UDGase, which is resistant to contamination, has high tabletop stability and batch-to-batch consistency, making it ideal for
biomedical applications and molecular diagnostics.
New Product 3: Applied Biosystems Platinum SYBR Green Master Mix (Available in 2023, coming soon)
Key features of Qiankun Platinum series qPCR master mix
1.
Sensitive: Both one-step multiplex and multiplex master mixes enable stable detection with lower lower detection limits (5 copies).
Figure 1.
Qiankun platinum one-step multi-mix sensitivity detection
.
5 copies and 10 copies of the artificial RNA template
were detected in the 20uL reaction.
Figure 2.
Qiankun Platinum Multi Master Mix Sensitivity Detection
.
5 copies and 10 copies of the artificial DNA template
were detected in the 20uL reaction.
2.
Multitasking: One-step multiplex and multiplex master mixes can achieve up to 5-fold target detection
Figure 3.
Qiankun Platinum One-Step Multiple Master Mix 5-stage test
.
The figure shows the linear regression results
of the 5-fold reaction of ACADM, APOE, GAPDH, RNaseP, and exogenous internal positive control (IPC) of human RNA using the Qiankun platinum one-step multiplex master mix.
Figure 4.
Qiankun Platinum Multi Master Mix 5-fold test
.
The figure shows the linear regression results
of the 5-fold reaction of human cDNA ACADM, APOE, GAPDH, RNaseP, and exogenous internal positive control (IPC) using TaqMan Platinum Multimix.
3.
Batch-to-batch consistency: The production process of Qiankun Platinum series master mix follows the ISO-9001 quality management system to ensure the consistency between different production batches
Figure 5.
Qiankun Platinum one-step multi-master mix: batch-to-batch stability
.
The one-step multiplex master mix amplification using 4 different batches of TaqMan Platinum includes single and multiplex reactions (up to 5 targets) for human gene expression and human or animal virus analysis
.
Excellent consistency
was observed in four batches.
Figure 6.
Batch-to-lot stability of Qiankun Platinum multi-master
mix.
The batch-to-lot consistency
of 5 different batches of TaqMan Platinum multiplex premixes between different gene expression targets and viral DNA targets was shown.
4.
Long duration, compatible with complex samples: In addition, Qiankun Platinum OneStep Multi Master Mix provides independent 10X enzyme master mix, 5X buffer system and ROX solution for users to quickly optimize
the reaction system.
At the same time, buffer components and proprietary additives have been optimized to ensure reliable results
even for complex samples such as saliva and crude cell extracts.
In addition, the Platinum OneStep Master Mix has an expiration date of up to 18 months, making it ideal for
developing robust assays.
Figure 7.
Qiankun platinum one-step method multi-master mixture crude extract applicability
.
The results of the detection of exogenous viral and endogenous human RNA targets in treated saliva and cellular crude extracts are shown
.
5.
Fast response, stable at room temperature: Qiankun Platinum TaqMan Multi Master Mix is compatible with rapid reaction mode, and can complete the reaction cycle
in 40 minutes on Applied Biosystems™ QuantStudio™ 5.
High stability - The prepared qPCR reaction system can be stably stored at room temperature for 72 hours
.
Figure 8.
Qiankun Platinum Multi Master Table Stability
.
The reaction system was completely established with different concentrations of ACTB target protein, human cDNA and master mix, and detected at 0 h and room temperature 72 h respectively, and TaqMan Platinum Multi Master Mix showed excellent desktop stability
.
Application scenarios of Qiankun Platinum series qPCR master mix
Applied Biosystems Platinum Series Master Mix is ideal
when you need sensitive or multiplex detection of pathogenic microorganisms in molecular diagnostics, qPCR experiments in biopharmaceutical R&D or quality control, or gene expression studies in basic research.
In the name of Qiankun, cast platinum quality - Applied Biosystems Qiankun platinum series qPCR master mix, to meet all the expectations
of Chinese users for high-quality master mix.
