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In the last lecture, we introduced the design of primer probes for digital PCR.
Template extraction
According to reports, digital PCR is compatible with various methods of extracting DNA and RNA templates in the laboratory, including the phenol chloroform method and various kit extraction methods
Quality Control
In order to obtain the best digital PCR performance, the purity and quality of the template should be checked
save
The storage of the extracted DNA template is also an important monitoring factor
Buffer and temperature conditions also affect the quality of the sample.
Other parameters inherent in the sample itself may affect the digital PCR experiment:
1.
If the template contains a GC-rich region, because the GC bond is very stable, this may result in incomplete amplification of the template
2.
Some manufacturers consider high-molecular-weight DNA or plasmids as an issue that affects partitioning
3.
Before starting the digital PCR test, you must perform: DNA copy number test conversion.
references
[1].
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