-
Characterization of Mycoplasmas by RAPD Fingerprinting
Time of Update: 2021-02-20
The diagnosis and typing of microorganisms of human and veterinary significance are vitally important in the rapid and effective treatment of infectious diseases.
Diagnostic microbiology laboratories are continuously updating methods in an effort to provide highly accurate, inexpensive, and rapid results for clinicians and research scientists.
-
Antigenic Typing of Field Isolates of Plasmodium by DNA Techniques
Time of Update: 2021-02-20
The frequency of the major mero- zoite surface antigen 1 (MSA-1) allelic forms in parasite strains iso- lated directly from patients varies dramatically from those found in in vitro cultured strains ( 6 , 7 ).
-
Studying Adaptation and Homeostatic Behaviors of Kinetic Networks by Using MATLAB
Time of Update: 2021-02-20
A particular interesting type of model relates to the so-called robust behavior where the homeostatic or perfect adaptation property is independent of the magnitude of the applied step-wise perturbation.
-
Storage of Mycobacterial Strains
Time of Update: 2021-02-20
The storage and maintenance of mycobacterial reference strains and clinical isolates are important parts of good laboratory practice in a mycobacterial laboratory.
The storage of reference and control strains facilitates a reliable control on intra- and inter-test reproducibility ( 1 , 12 ).
-
Mushrooms
Time of Update: 2021-02-20
fresh mushroom dehydrated into dried mushrooms, easy to transport and preserve, is an important north-south goods.
When cooking, the dried mushrooms should be made with water first.
, mushrooms tend to appear as a fresh.
-
Assessment of Multidrug Efflux Assemblies by Surface Plasmon Resonance
Time of Update: 2021-02-20
Here we describe the application of this method to study interactions of membrane proteins involved in multidrug efflux inEscherichia coli.
The SPR approach provides means to overcome some of these problems in studies of protein assemblies that function in the context of two membranes.
-
Analysis of Mitochondrial Gene Expression
Time of Update: 2021-02-20
Dictyostelium provides a well-established model system for the study of mitochondrial biology and disease.
Here we describe the methods deployed to study mitochondrial gene transcription and RNA processing inDictyostelium .
-
Propagation and Assay of BK Virus
Time of Update: 2021-02-20
BK virus (BKV), one of two human polyomaviruses, was first isolated in cell culture from the urine of an immunocompromised renal transplant patient in England in 1971 ( 1 ).
-
Transformation of Brucella Species with Suicide and Broad Host-Range Plasmids
Time of Update: 2021-02-20
Thus, the ability to transformBrucella with suicide plasmids (to introduce transposons or cause allelic exchanges) or replicating plasmids (to perform complementation studies) is paramount in implementing molecular approaches to unravel the genetic basis forBrucella virulence determinants.
-
30 Cases of Peptic Ulcer Treated by Dele
Time of Update: 2021-02-20
2 results 29 cases of symptoms in the experimental group disappeared, and X-ray and gastroscopic review of the original ulcer healed with 95% efficacy.
In the control group, only 18 cases of symptoms disappeared, ulcers healed, and the effect was 60%.
-
Immunoassay for Detection of IgA Antitissue Transglutaminase in Patients with Celiac Disease
Time of Update: 2021-02-20
Celiac disease is diagnosed by the demonstration of an altered intestinal mucosa in a jejunal biopsy specimen ( 3 ).
Serum IgA antibodies against endomysial antibodies (EMAs) are especially considered to be sensitive and highly specific markers for celiac disease ( 8 )( 9 ).
-
Extraction of RNA from Intracellular Mycobacterium tuberculosis: Methods, Considerations, and Applications
Time of Update: 2021-02-20
Pathogenicity inMycobacterium tuberculosis may be thought of as a multifactorial process with both pathogen and host-response effector molecules contributing to the process of infection, leading eit
-
Characterization of Specialized mtDNA Glycosylases
Time of Update: 2021-02-20
Mitochondria are cytoplasmic organelles that generate cellular energy in the form of ATP by the process of oxidative phosphorylation.
These few polypeptides encoded by mtDNA are essential components of oxidative phosphorylation.
-
Analysis of Cytomegalovirus Gene Expression by Transfection
Time of Update: 2021-02-20
Human cytomegalovirus (HCMV), a human herpesvirus, is the leading viral cause of birth defects ( 1 , 2 ).
HCMV has a severely restricted host range in culture.
-
Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants
Time of Update: 2021-02-20
Adenovirus mutants that lack essential genes must be grown by complementation, the products of the missing genes supplied by a source other than the viral genome.
-
Isolation of agr Quorum Sensing Autoinducers
Time of Update: 2021-02-20
Autoregulation of genes is often associated with quorum sensing systems where bacteria produce and secrete molecules that allow the cells to communicate with one another, leading to the activation of
-
Construction of Large Nave Fab Libraries
Time of Update: 2021-02-20
Na�ve libraries have been generated by tapping the natural primary (unselected) immune repertoire via cloning of Abs that recognize a variety of Ags ( 1 , 2 ).
-
Use of Proteomic Tools in Microbial Engineering for Biofuel Production
Time of Update: 2021-02-20
Hierarchical clustering analysis revealed that over 70% of the 102 differentially expressed proteins in mutant Rh8 were either upregulated (e.g., chaperones and solvent formation related) or downregulated (e.g., amino acid metabolism and protein synthesis related) in both acidogenic and solventogenic phase, which, respectively, are only upregulated or downregulated in solventogenic phase in the wild-type strain.
-
Micropipette Aspiration for Studying Cellular Mechanosensory Responses and Mechanics
Time of Update: 2021-02-20
Based on simple hydrostatic principles, this assay allows the application of a specific magnitude of mechanical stress on cells.
This powerful method has revealed insights about cell mechanics and mechanosensing, not only inDictyostelium discoideum but also in other cell types.
-
Engineering Escherichia coli for Canthaxanthin and Astaxanthin Biosynthesis
Time of Update: 2021-02-20
The carotenoid synthesis operons are assembled from genes isolated from carotenogenic bacterial sources.
Here, we describe methods to assemble carotenoid biosynthesis operons from carotenogenic gene clusters isolated from different bacterial sources and evaluate canthaxanthin or astaxanthin production inE.
