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Isolation of agr Quorum Sensing Autoinducers

 Last Update: 2021-02-20
 Source: Internet
 Author: User

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alpha cells secrete

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Autoregulation of genes is often associated with quorum sensing systems where bacteria produce and secrete molecules that allow the cells to communicate with one another, leading to the activation of certain genes at certain population densities. Here we describe the identification of the
agr
as a quorum sensing system in
Staphylococcus aureus
and the isolation of
agr
autoinducers and inhibitors by northern blotting, real-time RT-
PCR
, and β-lactamase reporter cells assays.

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