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Proteases Produced by Halophilic Bacteria and Archaea
Time of Update: 2021-01-25
Proteases from halophilic microorganisms present the advantage of being stable at high salinities, constituting interesting enzymes from a biotechnological point of view.
In this chapter, we describe the methods for the production of proteases by a representative organism from each of the two main groups inhabiting the saline habitats: the moderately halophilic bacteriumPseudoalteromonas sp.
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FRET-Based Biosensors for the Detection and Quantification of AI-2 Class of Quorum Sensing Compounds
Time of Update: 2021-01-25
LuxP-BAI2 binding induces changes in fluorescence resonance energy transfer (FRET) between CFP and YFP, whose magnitude of change is ligand concentration dependent.
This report describes the use of the LuxP-FRET biosensor for the detection and quantification of BAI-2.
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Effect of Helicobacter Pylori Eradication on Rebleeding of Duodenal Ulcer
Time of Update: 2021-01-25
2 Results gastric glasses confirmed that there was no significant difference in ulcer healing rates between the two groups of patients 4 weeks after the end of treatment.
We believe that hp-positive patients with hemorrhage of duoon ulcers should actively eradicate Hp treatment in order to reduce ulcer recurrence or re-bleeding.
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Differences Between Biogenic Amine Detection by HPLC Methods Using OPA and Dansyl Derivates
Time of Update: 2021-01-25
Although they are present in fermented foods and beverages in low quantities, they exhibit interactions with normal human metabolism (e.g., having vasoactive or psychoactive properties) that justify the research on their presence in foods and the possible related toxicological effects that they may cause.
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Modulation of Mammalian Cell Processes by Bacterial Quorum Sensing Molecules
Time of Update: 2021-01-25
Here we describe a set of western blot-based methodologies for the evaluation of biochemical effects specifically induced byN -(3-oxo-acyl) homoserine lactones (3-oxo-AHLs) small molecules secreted by a number of Gram-negative bacteria, including the opportunistic human pathogenPseudomonas aeruginosa .
First, we will highlight the AHL-mediated effects on proapoptotic and stress pathways.
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Bacterial Genomes for the Masses: Relevance to the Clinical Laboratory
Time of Update: 2021-01-25
Bacterial genome sequencing has revolutionized the research landscape and promises to deliver important changes to the clinical microbiology laboratory, through the identification of novel diagnostic
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Using the Baculovirus/Insect Cell System to Study Apoptosis
Time of Update: 2021-01-25
Apoptosis is a physiological program of cell suicide conserved in invertebrates and vertebrates.
Apoptosis is crucial to the normal development of organisms and in tissue homeostasis, by promoting elimination of unwanted cells including damaged- or virusinfected cells.
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Guide to Techniques in Creating Transgenic Mouse Models Using SV40 T Antigen
Time of Update: 2021-01-25
Moreover, by fusing a tissue-specific regulatory region of a particular gene to transforming genes, it is possible to direct expression of the transforming gene to specific cell types, thereby producing lines of mice that develop tumors in specific organs.
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Baculovirus Insecticide Production in Insect Larvae
Time of Update: 2021-01-25
Production of baculoviruses is generally done in larvae of a convenient host species, and the level of sophistication varies tremendously between field-collection of infected insects at the one extreme and automated mass manufacturing at the other.
First, the authors describe the production of wild typeHelicoverpa zea nucleopolyhedrovirus in larvae of the bollworm,H.
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Goan sharks
Time of Update: 2021-01-25
Dorsal fin 2, no hard thorns: the first dorsal fin is large, starting at the middle of the pectoral fin, and the second dorsal fin and hip fin are very small, the former is located above the front of the latter.
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Phage Transposon Mutagenesis
Time of Update: 2021-01-25
Mycobacterial transposon mutant libraries produced by ΦMycoMarT7 transduction are amenable to both forward and reverse genetic studies.
We then describe the transduction procedure and finally outline the isolation of individual transposon mutants.
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From Bacterial to Microbial Ecosystems (Metagenomics)
Time of Update: 2021-01-25
Metagenomic studies of microbial populations in different environments reveal the incredible diversity and adaptive capabilities of these organisms.
Here, we discuss the different components of a metagenomic study including sample collection,DNA extraction, sequencing, and informatics.
We conclude with examples of metagenomic studies conducted on environments of varying complexities.
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Comparison of three testing methods for Helicobacter pylori infection
Time of Update: 2021-01-25
In order to find a simple, inexpensive and easy experimental method, we tried to separate, identify and compare hp in gastric biopsy specimens with urea enzyme tests and smear lenses by candlestick, as reported below.
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Effect of Helicobacter Pylori Eradication on Rebleeding of Duodenal Ulcer
Time of Update: 2021-01-25
2 Results gastric glasses confirmed that there was no significant difference in ulcer healing rates between the two groups of patients 4 weeks after the end of treatment.
We believe that hp-positive patients with hemorrhage of duoon ulcers should actively eradicate Hp treatment in order to reduce ulcer recurrence or re-bleeding.
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Methodologies for the Analysis of Fungal Carotenoids
Time of Update: 2021-01-25
Carotenoids are high-value natural products utilized by several industrial sectors.
Some filamentous fungi such asPhycomyces blakesleeanus, Mucor circinelloides , andBlakeslea trispora possess the ability to synthesise carotenoids.
In this chapter, procedures for comprehensive analysis of fungal carotenoids are described.
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Immortalization of Human B-Lymphocytes by Epstein-Barr Virus
Time of Update: 2021-01-25
Epstein-Barr virus (EBV) is able to immortalize human B-lymphocytes with high efficiency.
First, EBV is the causative agent of infectious mononucleosis, which is a benign proliferation of B-lymphocytes ( 1 ).
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Analysis of DNA Sequences
Time of Update: 2021-01-25
Oligonucleotides can be labeled with radionucleotides or with chemiluminescent or fluorescent reporter molecules and used to probe nucleic acids extracted from samples ( 4 ), again giving qualitative or quantitative information regarding the occurrence of target organisms.
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Synthetic Genetic Array Analysis in Saccharomyces cerevisiae
Time of Update: 2021-01-25
For the yeastSaccharomyces cerevisiae , we developed a method termed Synthetic Genetic Array (SGA) analysis, which offers an efficient approach for the systematic construction of double mutants and enables a global analysis of synthetic lethal genetic interactions.
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Preparation of a Phage DNA Fragment Library for Whole Genome Shotgun Sequencing
Time of Update: 2021-01-25
Finally, examples will be given of how biological information about the phage genomic termini can be derived by analysis of theorganization of individual clones in the shotgun sequence assembly.
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Targeted Proteomics for Metabolic Pathway Optimization
Time of Update: 2021-01-25
We present here a targeted proteomics approach that uses selected-reaction monitoring (SRM) mass spectrometry to quantify multiple proteins in a sample.
SRM methods provide high selectivity and high sensitivity to enable rapid quantification of multiple proteins in an engineered pathway regardless of sequence or organism of origin.
