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Analysis of DNA Sequences
Time of Update: 2021-01-25
Oligonucleotides can be labeled with radionucleotides or with chemiluminescent or fluorescent reporter molecules and used to probe nucleic acids extracted from samples ( 4 ), again giving qualitative or quantitative information regarding the occurrence of target organisms.
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Immortalization of Human B-Lymphocytes by Epstein-Barr Virus
Time of Update: 2021-01-25
Epstein-Barr virus (EBV) is able to immortalize human B-lymphocytes with high efficiency.
First, EBV is the causative agent of infectious mononucleosis, which is a benign proliferation of B-lymphocytes ( 1 ).
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From Bacterial to Microbial Ecosystems (Metagenomics)
Time of Update: 2021-01-25
Metagenomic studies of microbial populations in different environments reveal the incredible diversity and adaptive capabilities of these organisms.
Here, we discuss the different components of a metagenomic study including sample collection,DNA extraction, sequencing, and informatics.
We conclude with examples of metagenomic studies conducted on environments of varying complexities.
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Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections
Time of Update: 2021-01-24
Methicillin-resistantStaphylococcus aureus (MRSA) is a major pathogen responsible for both hospital and community onset disease.
Resistance to methicillin inS .
aureus isolates and screening of patients for colonization with MRSA are important tools to limit the spread of this organism.
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Anti-intracellal bacterial infection immunity
Time of Update: 2021-01-24
However, after the activation of endocrine bacteria, M can secrete IL-12 and activate NK cells, NK cells bear an important early anti-cytobacterial defense function, can effectively kill and control cytobacteria infection.
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Detection of pathogenic fungi
Time of Update: 2021-01-24
General observation of fungal falles must be the fungus cultured in the sand to protect the weak legisic (commonly known as large culture), after several days to ten days of culture before you can see the typical bacteria.
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Myosome CCU determination
Time of Update: 2021-01-24
Lilac garden netizens "garden wood" to do mycosm CCU, and do not know how to choose the concentration of this initial bacteria liquid, because of the lack of standard 0.5 wheat concentration, so more
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Separation and culture identification of myogens
Time of Update: 2021-01-24
men: with male sterile swabs inserted into the urethra mouth 2-4 cm gently rotating secretions; If necessary, urine can also be used as myosome culture, take the first morning urine in the middle of 10-20 ml, after 2000rpm centrifugation for 10 minutes, take sediment inoculation, but the urine myophylline culture positive rate is significantly lower than urethra secretions.
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Semi-solid culture base transfer technique
Time of Update: 2021-01-24
(2) inoculated with sterile puncture, the needle is pierced into the center of the semi-solid fase at a depth of 0.5 cm from the bottom of the tube, and then exits in the same way.
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The preparation and counting of the culture base of Bacillus tuberculosis
Time of Update: 2021-01-24
- Qin culture base ingredients: hydrophosphate potassium 2.4g; a water magnesium sulfate 0.24g; magnesium acid 0.6g; Tianmen donglin 3.6g; 2% peacock green water solution 20ml; distilled water 600ml; potato starch 30g; glycerin 12ml; fresh egg liquid 1000ml.
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Yeast expression purification
Time of Update: 2021-01-24
Directly from the tablet to pick more clones, with MGY or BMGY culture -based culture, and then with BMMY culture induced expression, SDS -PAGE to detect whether the purpose protein is expressed, while selecting high-expression strains, while using Western-blotting to identify whether your purpose protein is ok.
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Immunocompetent, Semi-Permissive Cotton Rat Tumor Model for the Evaluation of Oncolytic Adenoviruses
Time of Update: 2021-01-24
In this chapter we describe an immunocompetent, semi-permissive cotton rat tumor model to evaluate the safety and efficacy of oncolytic Ad vectors.
We present methods for generating subcutaneous tumors in cotton rats and assessing the efficacy of Ad vectors upon intratumoral injection.
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Separation screening of bacteria produced by industrial microorganisms (I)
Time of Update: 2021-01-24
Forest soil on hillsides, thick vegetation, dead branches and leaves, rich organic matter, and dark and humid, suitable for mold, yeam bacteria growth and reproduction, the number of microorganisms correspondingly less.
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What are the advantages and disadvantages of NEB's M13 phage random peptide library and NOVAGE's T7 phage peptide library?
Time of Update: 2021-01-24
fusion sequence size: M13 phage display peptide library for 7 peptides, 12 peptides and ring 7 peptides, amino acids ; T7 phage display peptide library prefab: 11329 yuan; product use: NEBM13 phage display peptide library can be used to locate antigen decision clusters, determine protein interaction points, identify enzyme substrates or inhibitors, Identification of subject activators or inhibitors, development of peptide drugs, development of new drugs, tumor therapy and vaccine manufacturing, a wide range of uses.
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Observation of the basic form and disease caused by the bacteria
Time of Update: 2021-01-24
(2) layer rust genus (Phakopsora) winter spores without handle, single cells, each other up and down the side of each other secretly formed a multi-layer, cushion-like, buried under the host skin, take the P.ziziphi-vulgaris specimen observation symptoms will point, and mirror the bacteria preparation 225, pay attention to the above-mentioned morphological characteristics, and the difference with no handle rust.
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The pattern of line bacteria was observed by cellopic agar flat dialysis culture
Time of Update: 2021-01-24
celloprotein has semi-permeable membrane characteristics, its light transmission and slide is basically the same, the use of celloprotein agar flat dialysis culture, can make the line bacteria grow on the celloprotein, and then the long bacteria of celloprotein cut small pieces, attached to the slide, with scope mirror can be seen to the natural growth of the individual form of the line bacteria.
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Microorganisms are commonly used in the preparation of reagents and solutions
Time of Update: 2021-01-24
, 3% acidic alcohol solution strong hydrochloric acid 3 ml 95% alcohol 97 ml 2, neutral red indicator neutral red 0.2 04 g 95% ethanol 28 ml distilled water 72 ml neutral red pH6.8-8, color from red to yellow, common concentration of 0.04%.
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Media for Marine Microbes: Marine Agar 2216 (DSMZ Medium 604)
Time of Update: 2021-01-24
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L.
Distribute into tubes or flasks.
Pour into sterile Petri dishes or leave in tubes.
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Two methods of bacterial counting
Time of Update: 2021-01-24
suncg gives a standard count of bacteria: standard practice is to add quantitative bacterial suspension (1 ml, 0.5 or 0.1 ml) to melted to 50-55 degrees agar culture base (generally do bacterial fall count with nutritional agar plate, and do fillfill count with sass flat) after mixing in the incubator observation.
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Botox testing in food
Time of Update: 2021-01-24
I. Experimental principle the sample after the sterilization of the flat plate to isolate the single bacterios, pick suspected bacteria fell to trypsin glucose yeast-soaked broth (TPGY) culture -base culture, dna extraction for the culture reagement box to extract DNA, PCR amplification, agar sugar gel electrophoresis to test whether the PCR products contain Botox characteristic strips, so as to initially determine whether food is contaminated with Botox.
