Semi-solid culture base transfer technique
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Last Update: 2021-01-24
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Source: Internet
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Author: User
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is used
to preserve
and indirectly check for bacteria for motivation.(1) the material
semi-solid
culture
base.
a bacterial bevel culture.(2) method
(1) the inoculation needle is cooled by flame sterilization and bacteria are removed from the surface of the slope culture.
(2) inoculated with sterile puncture, the needle is pierced into the center of the semi-solid
fase
at a depth of 0.5 cm from the bottom of the tube, and then exits in the same way. Be careful to keep the needle from swinging outside the puncture line when stabbing in and out.
(3)
test tube
through the flame sterilization, stuffed with cotton plugs. The inoculation needle is sterilized by flame. The culture is cultured in a
at a constant
of 37 degrees C.(3) results
the most dynamic bacteria spread and grow, and the undying bacteria grew along the puncture line.
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