Separation and culture identification of myogens

Uu and human mycoma (Mh) are common pathogenic urinary tract myosomes. Depending on the my myliogen,
reaction characteristics
resistance to a particular substance can be used to isolate and identify mytrophysics.of the
are inoculated in my myocytic identification and culture
the
of the disease. The lysolycogen can produce arginase, which produces ammonia from sugar, and the culture is alkaline, and the indicator changes from yellow to pink. Human-type mycomogens can break down arginine to produce nitrogen, so that the culture containing arginine broth is alkaline and pink.the material
a primary separation identification culture.the collection of
specimens should be sent for examination immediately, room temperature preservation shall not exceed 2 hours, and room temperature shall not exceed 5 hours.
men: with male sterile swabs inserted into the urethra mouth 2-4 cm gently rotating secretions; If necessary, urine can also be used as myosome culture, take the first morning urine in the middle of 10-20 ml, after 2000rpm centrifugation for 10 minutes, take sediment inoculation, but the urine myophylline culture positive rate is significantly lower than urethra secretions.
: after using a vasomizer to enlarge the yin, use a female swab in the cervical mouth 1-2cm gently rotate to take cervical secretions; Urine is not recommended.the culture
1, the number of culture tubes needed for the reheat of the culture tube, reheat to room temperature, make a good mark.
2, inoculation will be swab inserted into the culture tube, rotation and squeeze several times, so that the swab in myosome seep into the liquid, swab after sterilization treatment properly handled;
3, culture 35-37 degrees C
thermostat
24-48h hours, observation results.results
Uu should be reported within 24 hours, Mh should be reported within 48 hours, and other results should be reported within 48 hours, up to a maximum of 72 hours. The culture tube liquid changes from orange to red, transparent and bright, no obvious turbidity, indicating that there is Uu and/or Mh growth, can be judged positive; If necessary, we can do mycoplibacterial solid culture, low-fold mirror observation of the growth of lotus egg-like bacteria for mycoplibacteria positive.Note

1, Uu, Mh growth should keep the culture fluid transparent and clear, no obvious turbidity, occasional turbidity should not report myophyloid positive.
2, Uu growth, culture normal color change time in 24 hours, but if the specimen content is small, the color change time may be more than 24 hours. Mh growth, culture normal color change time within 24-48 hours, if the specimen content is large, the color change time may be less than 24 hours.
3, can not be sure whether there is clutter pollution, the culture fluid can be removed a little inoculated on the plate or through the 0.45um membrane
filtering
culture, according to the blood plate bacteria growth or filter culture color changes to judge the clutter pollution.