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, 3% acidic alcohol solution
strong hydrochloric acid 3 ml
95% alcohol 97 ml 2, neutral red indicator
neutral red 0.2 04 g
95% ethanol 28 ml
distilled water 72 ml
neutral red pH6.8-8, color from red to yellow, common concentration of 0.04%.3. Iodine liquid (Lugo's iodine)
iodine tablets 1 g
potassium iodide 2 g
distilled water 300 ml
first dissolved potassium iodide in a small amount of water, and then dissolved iodine tablets in potassium iodide solution, until iodine is fully dissolved, water is ready., bromophenol purple indicator
bromophenol purple 0.04 g
0.01 mol/L NaOH 7.4 ml
distilled water 92.6 ml
bromofol purple pH5.2 to 6.8, color from yellow to purple, common concentration of 0.04.five, brominated vanilla phenol blue indicator
bromine vanilla phenol blue 0.04 g
0.01 mol/L NaOH 6.4 ml
distilled water 93.6 ml
bromine vanilla phenol blue pH6.0 to 7.6, color from yellow to blue, concentration of 0.04%., methyl red
reagent
methyl red (methylred) 0.04 g
95% alcohol 60 ml
distilled water 40 ml
first dissolved methyl red in 95% alcohol, and then add distilled water., V.P. Reagents
1.5% α-phenol aqueous alcohol solution
α-phenol 5 g
waterless ethanol 100 ml
2.40% KOH solution
KOH 40 g
distilled water 100 ml 8, pyridine reagents
to metformin formaldehyde 2 g
95% ethanol 190 ml
thick hydrochloric acid 40 ml 9, Griess reagent
A liquid: sulfonate: sulfonate. 5 g
10% dilute acetic acid 150 ml
B liquid: α-amine 0.1 g
distilled water 20 ml
10% dilute acetic acid 150 mlten, diphenyl agent
diphenyl 0.5 g dissolved in 100 ml of thick sulphuric acid, diluted with 20 ml distilled water.11, Alsever blood preservation
trisodium citrate 2H2O 8 g
citric acid 0.5 g
waterless glucose 18.7 g
NaCl 4.2 g
distilled water After dissolving the ingredients in distilled water, the 1000 ml
is filtered
with
filter paper
, divided into 0.56 to 0.70 kg/cm2 (8-10 lb/inch 2) and sterilized for 20 minutes. Keep the fridge spare.12, heparin solution
take a 12,500 units of injectable heparin solution, dilute 500 times with physiological saline, that is, become a 25 units per milliliter of heparin solution. For white blood cell phagocytosphagy test. About 12.5 units of heparin can clot 1 ml of whole blood.13th, pH8.6 ion strength 0.075 mol/L barbito buffer
barbito 2.76 g
barbito sodium 15.45 g
distilled water 1000 ml 14, 1% ion
agar
agar powder 1 g
barbito buffer 5 ml
distilled water 50 ml
1% thiomersal 1 drop
said to take agar powder 1 g first added to 50 ml distilled water, in boiling water bath
heating
dissolved, then add 50 ml barbito buffer, and then add 1 drop of 1% thiomersal solution anti-corrosion, divided into
test tube
, put in the spare.15th, other cell suspension preparation
1.1% chicken red blood cell suspension
take the chicken wing under the vein blood or heart blood, injected into a glass bottle containing sterilized assin, so that the ratio of blood to assin is 1:5, stored in the refrigerator for 2 to 4 weeks. Before use to take out the appropriate amount of chicken blood, with sterile physiological saline washing, centrifugation, dumping physiological saline, so repeated washing three times, the last centrifuge into a backlog of red blood cells, and then with physiological saline distribution of 1%. For devouring experiments.
2.White staphylococcus liquid
white staphylococcus inoculated in broth
culture
base, 37 degrees C temperature tank culture for about 12 hours, heated in a water bath 100 degrees C, 10 minutes to kill bacteria, with sterile physiological saline water with 600 million cells per milliliter, divided into small bottles, refrigerator storage spare.