Media for Marine Microbes: Marine Agar 2216 (DSMZ Medium 604)

Composition per liter:
NaCl …… 19.45g
Agar …… 15.0g
MgCl2…… 8.8g
Peptone…… 5.0g
Na2SO3…… 3.24g
CaCl2…… 1.8g
Yeast extract …… 1.0g
KCl…… 0.55g
NaHCO3 …… 0.16g
Ferric citrate…… 0.1g
KBr…… 0.08g
SrCl2…… 0.03g
H3BO3 …… 0.02g
Na2HPO4 …… 8.0mg
Na2SiO3…… 4.0mg
NaF…… 2.4mg
NH4NO3 …… 1.6mg
pH 7.6 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Diagnostic Systems.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Hyphomonas spp., Oceanospirillum spp., Hyphomicrobium indicum, Psychroflexus gondwanensis=Flavobacterium gondwanense, Salegentibacter salegens=Flavobacterium salegens, Psychromonas antarctica, Sulfitobacter mediterraneus, Thalassomonas viridans,Vibrio spp., Marinospirillum minutulum=Oceanospirillum minutulum, Terasakiella pusilla=Oceanospirillum pusillum, Pseudoalteromonas atlantica=Alteromonas atlantica, Pseudomonas atlantica,Roseobacter spp., Erythrobacter longus, Pseudospirillum japonicum=Oceanospirillum japonicum,Marinobacter hydrocarbonoclasticus （Pseudomonas nautica）, Psychrobacter spp., and Moritella japonica.
For the isolation, cultivation, and maintenance of a wide variety of heterotrophic marine bacteria.