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The pattern of line bacteria was observed by cellopic agar flat dialysis culture
Time of Update: 2021-01-24
celloprotein has semi-permeable membrane characteristics, its light transmission and slide is basically the same, the use of celloprotein agar flat dialysis culture, can make the line bacteria grow on the celloprotein, and then the long bacteria of celloprotein cut small pieces, attached to the slide, with scope mirror can be seen to the natural growth of the individual form of the line bacteria.
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Separation and culture identification of myogens
Time of Update: 2021-01-24
men: with male sterile swabs inserted into the urethra mouth 2-4 cm gently rotating secretions; If necessary, urine can also be used as myosome culture, take the first morning urine in the middle of 10-20 ml, after 2000rpm centrifugation for 10 minutes, take sediment inoculation, but the urine myophylline culture positive rate is significantly lower than urethra secretions.
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Single Cell Microfluidic Studies of Bacterial Motility
Time of Update: 2021-01-24
It is proposed that motility is produced when surface immobilized Agl-Glt complexes produce traction on a rigid track, possibly the MreB cables.
These approaches require a microscopy chamber where the cells glide in liquid on a non-agar substrate.
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The method of total yeast RNA extraction
Time of Update: 2021-01-24
(1) the yeast cells cultured in 3 ml selective medium , 30 degrees C overnight rotation culture.
(9) to take the water phase and add the same volume of phenol/chloroform/isoquinol (25:24:1), 10000rpm/min centrifugation 8min.
(10) to take the water phase and add iso-volume chloroform/isoquinol (49:1)10000rpm/min centrifugation 8min.
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Culture base preparation techniques
Time of Update: 2021-01-24
2, used glassware Where there are no pathogens or utensils not contaminated with bacteria, after use can be flushed at any time, absorbed chemical reagents straw, can be soaked in clean water, until a certain number of then concentrated cleaning.
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Questions and answers for myophysics cultivation
Time of Update: 2021-01-24
arginine myganic broth culture is used to cultivate oral myganics, the use of arginine redness, then there is the growth of oral myganos, if you want to separate, then inoculated to arginine myganic agar media.
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Overview of disinfection and sterilization
Time of Update: 2021-01-24
There are many methods of disinfection and sterilization, which can generally be divided into heating , filtration, irradiation and the use of chemicals.
with dry heat sterilization and autobaric steam sterilization will be destroyed, you must use intermittent sterilization method.
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The basic noun explanation of microbiology
Time of Update: 2021-01-24
nuclei microbe (prokaryotic microbe) : refers to the nucleosis and cytomegame does not exist between the distinct nucleofilm, its chromosome is composed of a single nucleic acid of a class of microorganisms.
bacteria (bacterium) : single- or multi-celled tiny primary nuclear organisms.
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Analysis of Linker Histone Binding to Mono- and Dinucleosomes
Time of Update: 2021-01-24
The assembly of DNA into the eukaryotic nucleus via an ascending hierarchy of intermediate chromatin structures has two major functional consequences: 1.
The intermediate chromatin structures provide a filing system that greatly facilitates the search by RNA polymerase for regulatory DNA and the associated trans-acting factors.
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Green (p.aeruginosa)
Time of Update: 2021-01-24
The primary endotoxin protein is a highly molecular, low toxicity, immunogenic protective antigen, not only found in different sexual type of green pus bacteria, but also widely found in other bacteria of the genus Pneumococcal, E.
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Morphological observation of fungi from plant diseases
Time of Update: 2021-01-24
(1) Microcycle: The microcycle is a kind of sleeping body formed by thin wall tissue and loose silk tissue, which is not only the organ of fungus storage nutrients, but also the sleeping mechanism used by fungi to get through the bad environment, the nucleus size is different, the color and shape are different.
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Phage amplification, titration measurement method
Time of Update: 2021-01-24
day 17:00: After inoculation ring to take a monoclonal bacteria inoculated in LB culture liquid, 37 degrees C shaker overnight (no more than 16 hours) second Day 7:30: Turn on the UV light and s
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Purification and visible of the KERP complex of influenza A virus
Time of Update: 2021-01-24
0:00 A influenza virus keruclein complex purification and visible 0 0:58 Content Introduction 58 1:27 Cleavage influenza A virus 87 2:53 glycerin gradient semoulation rate centrifugation 173 4:18SDS
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Things to cluster people to bacteria
Time of Update: 2021-01-24
"We don't have research subjects from remote mountain villages." the discovery of "intestinal type" stems from a perennial study of the human body's fine species , the so-called gut microbiome group- of the body , which is an extremely difficult task because each person has nearly 100 trillion bacteria in their body (you know, the number of cells in the body itself is no more than 10 trillion).
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Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates
Time of Update: 2021-01-24
The method involves running a first-dimension gel in order to separate restriction-digested replication intermediates of different mass.
The 2-D gel is then blotted and probed to allow an examination of replication intermediates in specific DNA regions.
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Canine Adenovirus Downstream Processing Protocol
Time of Update: 2021-01-24
Vectors derived from canine adenovirus type 2 (CAV-2) are among the best-studied representatives.
A detailed protocol describing a complete scalable downstream processing strategy for CAV-2 vectors is reported here.
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Beef paste 胨 preparation of a culture base
Time of Update: 2021-01-24
4—7． 6 , equipment beef paste, protein , NaCl, agar; 1mol/L NaOH, 1mol/L HCl; test tube, triangular flavon, beaver, drum, glass rod, medium splitter, torque balance , horn spoon, auto-steam sterilization pot, pH test paper (pH 5.
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Humble bacteria are as warm as jade
Time of Update: 2021-01-24
hate is not born at the same time, day and day with bacteria good.
Peng Ruibao: a few degrees of temperature control, bacteria look good.
Hua Liangxuan's hair cloud: The mountain circuit does not turn to bacteria T T...
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Virus purification method and virus titration measurement method
Time of Update: 2021-01-24
For routine applications however, we titrate the virus in a monolayer assay on Vero cells, which normally results in the appearance of large, easily counted plaques.
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Clinical Microbial SOP
Time of Update: 2021-01-24
7． Close the air valve or cut off the power supply, let the pot items cool naturally, do not immediately open the exhaust valve, to avoid accidents.
