Questions and answers for myophysics cultivation

, Introduction:
primary body size of 0.1 to 0.3 m, can pass
filtering
bacteria, often to cell
culture
work to bring pollution trouble. Small (diameter 0.1 to 1.0 mm) and
"
" on solid
culture base. Cell wall-free, can not maintain a fixed shape and presents polygonality, sensitive to osmotic pressure, insensitive to
antibiotics
cell wall synthesis.
is not easy to color, so it is commonly used Giemsa dyeing to dye it lilac. Cell membrane cholesterol content is more, about 36%, to maintain the integrity of the cell membrane has a certain role. Cell membranes contain sterols and are tougher than those of other primary nuclear organisms. Any substance that can act on cholesterol (e.g. b, saponin, etc.) can cause the destruction of mycomycin membrane and cause mycomal death.
a single primary genome with double-stranded DNA with a small molecular weight (only one-fifth of E. coli) and limited synthesis and metabolism.
end of the mycules of pneumonia has a special end structure (terminal structure) that enables the mycosms to adhere to the surface of the epithelial cells of the mucous membranes of the respiratory tract, which is related to pathogenicity.
bio-chemical reactions and parting:
can generally break down glucose mycoma can not use arginine, can use arginine can not break down glucose, according to which mycomogen can be divided into two categories. Glucose or arginine cannot be used by the dexterity myophylactics, but urea can be used as an energy source.
all kinds of myganics have specific surface
antigen
structure, very few cross-reaction, with type specificity. The growth inhibition test (GIT), metabolic inhibition test (MIT) can identify mycogen antigens for parting.
it is widely distributed in nature, there are more than 80 species. Human-related mycomals include pneumonia mycombines (M-pneumonie, Mp), human-type mycombinos (UU decomposing urea mycombinosomes), and genital mycoma (M.genitalium, MG).
caused by my myophysics of pneumonia. Seven myosomes have been isolated from the human genitourinary tract, of which the high separation rate is related to the genitourinary disease, which is the dexterity mysyl, followed by the human-type myosome. Human myosomes (M.humenis, MH), Ureaplasma urealyticum, UU, and genital myosomes (M. genitalium, MG) can all cause urinary tract infections.
is widely found in the natural world to grow cholesterol, genetically independent of bacteria, and under no conditions can it become smaller bacterial bacteria, 0.1 to 0.3 mm.
conditions:
nutritional requirements are higher than that of general bacteria, in addition to basic nutrients, 10 to 20% of people or animals
serum
needed to provide myalgic cholesterol. The most suitable pH7.8 to 8.0, below 7.0 death, but the most suitable pH 6.0 to 6.5.
anaerobic, and some strains grow better with 5% CO2 at the beginning of separation. Slow growth, incubating on solid cultures with less
agar
content for 2 to 3 days a typical "lotus egg-like" bacterium: round (diameter 10 to 16 m), thick core part, down into the culture base, surrounded by a thin layer of transparent particle area. In addition, myophylogens can grow in chicken embryo fluffy urethra membranes or cultured cells.
, FAQ:
1, hello, I would like to do my mydicinal testing according to the pharmacopeia 2010 three parts, what media are needed? What is the role of arginine myganic broth media? Pharmacopeia said to use mycelin broth media, would like to ask whether with arginine, there is a myganic agar media is required to contain arginine? Are we testing to see if our cultured cells are mycular contamination?
:
1) Pharmacopeia 2010 Three myophylogen testing requires myophyl semi-fluid media and mygan broth media (or mycelin agar media).
For validation test: mycosm broth media is used to culture mycoids of pneumonia, the use of glucose yellowing, then there is the growth of myculins of pneumonia, if you want to separate, then inoculated on myceloid agar media.
arginine myganic broth culture is used to cultivate oral myganics, the use of arginine redness, then there is the growth of oral myganos, if you want to separate, then inoculated to arginine myganic agar media.
2) Myophylactic broth media and myogen agar media do not contain arginine.
3) myosome growth phenomenon is to observe whether the liquid is changing color, cultured liquid is still clarified, not turbid growth, if cloudy growth, indicating that there is bacterial contamination, normal is to inhibit bacterial growth.
2, want to separate myosomes. Don't know how to choose a culture base?
: First of all, you should determine which myosome to separate. Do you use glucose, arginine, or urea?
Use of glucose mycosphones: pneumonia mycosphone ---- mylophon broth mediums---- The use of myumogens of urea: T mysyl (de-lysing myganics—-- de-diastocin media chicken toxabinogens------ improved Frey media for solid culture: the above myalges can be inoculated on myalge agar media.
3, improved Frey media is directly used to cultivate my mysyl?
: For the cultivation of fluid mycules and other poultry mycules.
4, if there is a mycosm, is it an improved Frey media liquid that changes color?
: Yes, the modified Frey medium contains glucose and phenol red, which produce acid when mycoploids use glucose, resulting in a decrease in pH, yellowing of the solution color, and clarification of the solution.
5, we are used to cultivate goat myonogen, has not been cultivated.
: May not have enough culture conditions, the serum quality is not good, CO2 concentration is 5%-10%, N2 concentration is 95% growth is good.
6, hello, I just searched your website for my myogen media and found that there are a variety of myophylogen media 100 ml 600 yuan, but my myalge culture base is 250 g 300 yuan. Can the base of myophysics cultivation be used to cultivate the anti-lysophysium.
: Yes, our anti-mycodes are produced finished media that can be used directly.
7, hello, I would like to ask, I match the media support primary pink is what is the reason?
: The media contains phenol red, used to observe the growth of mycophon? If the color change and the solution is clarified, it can be judged to have mycosome growth.
8, do you have a tablet of my myonogenic media?
: It is recommended that you make your own, because the cultivation time is long, the tablet is prone to water loss.
9, hello, our company recently do mygen testing,
PCR
method, but the pharmacopeia method also want to do a look at the pharmacopeia mycenae testing method, see your family has myonogen broth medium, want to ask, your company for your products have carried out mygan broth medium sensitivity testing at the same time, the previously specified medium is designated by the Beijing Central Inspection Institute, but they do not have myganic broth medium, but there are other media for related mygans.
: A sensitivity test was conducted, the pneumonia myogen reached 10-8 chromic units, and the oral myalgen reached 10-4 chromic units.
10, consult, mycenadra broth culture for the myalgesics there are several? Or can all myosomes be cultured?
: the use of glucose mycosomes, such as pneumonia mycosms.
11, myophysics general training generally take how long?
: myophysics culture time is relatively long, fast-growing T myophytes need to be cultured about 1-3 days. Medium growth takes about 7-14 days and slow takes about 21-30 days.
12, want to buy pneumonia myophylitis media, HB7025-3 myalges agar media, is it ability to cultivate pneumonia myophyllin?
: HB7025-3 primary agar media, is a myophyl solid plate culture method, can cultivate myalpines and oral myalpines of pneumonia.
"Penicillin 800,000 units" in the medium use of 13, HB7025-2 primary broth mediums is not clear.
: Penicillin 800,000 units, refers to penicillin price units, not weight units. Instructions are available on the packaging when purchasing penicillin.
14, the culture of chicken toxic myonogen and pig pneumonia myophylla with which media is better?
: Culture chicken toxolycogens with improved Frey media, culture pig pneumonia mycenos with myalges or pig mycenos culture.
can I provide some information on the 15th, and the culture base used with PPLO broth? I now need to resuscitate and pass on my pneumonia myonos.
: Recovery can be made with sp4 glucose broth culture base, passed on sp4 glucose agar culture base.
16, ask the myophysics solid media, which can detect the cogens of chickens?
: Yes.
17, want to
cell culture
process, whether there is myocytostic contamination, which media can be used?
: Yes, we can use mycenae broth media and mycenadra semi-fluid media.
changes in the color of myo18 and myophysics growth media?
: Mycoplasphonsin broth medium and arginine mycoplasphons broth medium formulations contain phenol red. If a myophylactic grows, the media pH decreases or rises, so the media color becomes yellow (mycelin broth media) or red (arginine myganic broth media).
19, myophylactic media base this contains arginine and no arginine what difference, we want a broad spectrum of myotoplasm media, recommended to use arginine or arginine-free?
: Broad-spectrum mycopheric media can not use a media containing phenol red, can use mycopheric semi-fluid media or mycoplium agar media.
20, mycomal media base is to add horse serum, penicillin those or mycomal additives ah?
answer: mycomal media base does not contain horse serum and penicillin, need to add horse serum and sterile penicillin solution after the basic medium autocultension.
21, is there a mycobacterial strain?
: No.
22, please ask, do you have a chicken poison mygenic base media?
: Yes, improve Frey's culture.
23, I look at the company's several mygenic media, the composition is different, how exactly?
answer: For mytrogens from different sources, different forms of myalges culture, which is conducive to the growth and cultivation of mygenes from different sources.
24, which is the media used for cattle and sheep mysons?
: HB8572-2 primary media, Chinese veterinary drug standards.
25, your company has tested the poultry vaccine mygenic media?
: Yes, improve FREY's culture.
26, please ask your myalic base media after the matching can identify myalges?
: No, you can do some bio-chemical identification, such as glucose, arginine, urea, TTC reduction, red blood cell adsorption and other tests.
27, I see you have a variety of myalge media, I do not know how to buy that I see other people do my mygenic culture, first with liquid, and then with solids. I bought solids directly last time, and I'm going to grow my myons, and I need to grow them. With liquid, need to buy that?
A:1) Myophyloid broth media is used to culture mycoids of pneumonia, the use of glucose yellowing, then there is the growth of myculins of pneumonia, if you want to separate, then inoculated to myceloid media. Arginine myganism broth media is used to culture oral myganism, the use of arginine redness, then there is the growth of oral myganos, if you want to separate, then inoculated to arginine myganic agar media.
28, hello, you have there useful to do myonogeneic bio-chemical identification of the media?
: Yes, you can do
1) TTC reduction test: pneumonia mycobacteria is pink or crimson;
2) glucose, arginine, urea test;
3) 3% red blood cell adsorption test: red blood cells with chickens, wherein pneumonia mycotoplasm, oral mycobacterium I and III type adsorption, positive.
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