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Yeast culture method
Time of Update: 2021-01-24
Yeast can reproduce asexually through germination or by forming cystic spores.
cultured yeast with the most suitable pH value of pH4.5-5.0.
The cultures used are: Yeast Full Culture Base YPD: Yeast Extract 10g, Protein Chen 20g, Glucose 20g, fixed to 1L.
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Preparation of Gaussi 1 culture
Time of Update: 2021-01-24
For trace ingredients such as FeSO 4.7H2 O, a high concentration of storage fluid can be pre-arranged to add a certain amount as needed in the preparation of the culture base.
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Observation of plant pathogenic bacteria and their pathogenic symptoms
Time of Update: 2021-01-24
rice white leaf disease (Xanthomonas oryzae) cotton bacteria horn plaque disease (X.malvacearum) potato cyclosis (Clavibacter sepedonicum cabbage soft rot (Erwinia carotovora) cucumber bacteria horn plaque disease (X.malvacearum) apple root cancer (Agrobac) apple root cancer (Agrobac).
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HSV-2 virus culture question and answer
Time of Update: 2021-01-24
Blind transmission is in the case of do not know whether there is a virus, or in the amount of poison is very small, and you can not be sure, still receive the poison, and then continue to receive the poison, consecutive batches, if the poison will appear CPE, if not will not appear.
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D'Lee treated 36 cases of gastrointestinal disease
Time of Update: 2021-01-24
Ulcer healing: After 1 course of treatment, 7 patients with stomach ulcers were cured and 1 person improved.
Dele punch can eliminate HP, so the drug is good for the healing of ulcers, the cure of gastritis, or to avoid the recurrence of peptic ulcers.
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Recombination of E. coli fermentation tank culture
Time of Update: 2021-01-24
Adjust the cursor to DO gear, set the parameters to 10/20/30, adjust to the oxygen-dissolved joint detection.
2) to adjust the cursor to pH gear, set the parameter 7.0, adjust to automatic detection.
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Detection of microbial growth
Time of Update: 2021-01-24
abstract: the detection of microorganisms is of great significance in both theoretical research and production practice, and the method of growth measurement, microbial counting, physiological index method and commercial rapid microbial detection briefly introduces more than 20 commonly used detection methods using microbial weight, volume, size, physiological metabolites and other indicators, and briefly introduces the principles, application scope and advantages and disadvantages of these methods.
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Lyovirus packaging and use of research essential work manual applications
Time of Update: 2021-01-24
slow virus packaging and use of utilities and tips (Chinese third edition) fresh out!
National Free Technical Service Hotline: 400-188-0198, 86-21-50793722-606 Slow Virus Spot Product Promotion: Email: sunbio@sbo-bio.com.cn
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IMVIC and hydrogen sulfide test
Time of Update: 2021-01-24
coli-producing bacteria punctured into 2 lead acetate mediums, 37 degrees C greenhouse culture for 48 hours.
(3) inoculated the above-mentioned bacteria in 2 glucose water mediums (do methyl red test and volt-pu test) and cultured in a greenhouse at 37 degrees C for 2 days.
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The effect of external factors on bacteria
Time of Update: 2021-01-24
(2) circulating steam sterilization method: the use of about 100 degrees C of water vapor disinfection, generally using circulating steam sterilizer (the principle is equivalent to China's steamer), heating 15 to 39 minutes, can kill bacterial reproduction.
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Method for determining the total number of falling bacteria
Time of Update: 2021-01-24
Sa standard tablet culture counting method although the total number of bacteria developed by the state measurement method, to a certain extent can reflect the health quality of food, but some food can not make an accurate evaluation, because the bacteria adapt to the growth of high temperature, moderate temperature and low temperature, the required pH and nutritional conditions are not the same, and culture time is also related.
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Virus purification method and virus titration measurement method
Time of Update: 2021-01-24
For routine applications however, we titrate the virus in a monolayer assay on Vero cells, which normally results in the appearance of large, easily counted plaques.
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Clinical Microbial SOP
Time of Update: 2021-01-24
7． Close the air valve or cut off the power supply, let the pot items cool naturally, do not immediately open the exhaust valve, to avoid accidents.
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Bacterial whiplash staining
Time of Update: 2021-01-23
(i) Experimental purpose: to learn the bacterial whiplash dyeing method (ii) experimental principle the bacteria whip hair is very fine, the diameter is generally 10-20nm, only with electron scope can be observed.
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Bio-chemical reactions commonly used in microbial identification
Time of Update: 2021-01-23
Place in a 37-degree C thermostat to culture 24h, after removal to observe the color of flat moss, if red spots appear, that is, the fat is hydrolysed, this reaction is positive.
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Diluted plate measurement method
Time of Update: 2021-01-23
First, the experimental purpose to understand the principle of dilution plate counting, master the application of flat plate culage method and mixed plate culture method, to understand bacteria, line bacteria, mold fall characteristics.
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The characteristics of myalgenes and the collection of specimens
Time of Update: 2021-01-23
I, the character pattern and structure the size of the primary body is 0.2 to 0.3um, can pass filtering bacteria, often to cell culture work to bring pollution trouble.
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Instructions for the use of color-showing substation kits (end-point color-showing method, with ad parity reagents)
Time of Update: 2021-01-23
(4) experimental operation take the thermogen-free test tube, add 100 ml of bacterial endotoxin to check water, endotoxin standard solution, or for test products.
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Problems related to glass beads in yeast granulation extraction
Time of Update: 2021-01-23
1: In the extraction of yeast particles, there is a step to use glass beads, who can tell me the role of glass beads, and where can buy glass beads, expensive?
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Potassium cyanide (KCN) culture base
Time of Update: 2021-01-23
test method inoculated agar cultures in protein water to become diluted bacteria, and 1 ring was selected to be inoculated in potassium cyanide (KCN) culture.
