Preparation of Gaussi 1 culture

The preparation of the 20 Gao's No. 1
Culation
base I, the purpose requires through the preparation of Gao's No. 1
lechration base
to master the general method of the preparation of synthetic culture., basic principle The Gaussia 1 culture base is a synthetic culture base for separating and cultured line bacteria. This culture is made up of soluble starch (as a carbon source), KNO3 (as a nitrogen source), NaCl, K
2
HPO
4
.3H
2
O, MgSO
4
.7H
2
O (as inorganic salt, providing sodium, potassium, phosphorus, magnesium, sulfur plasma for
microorganisms
) and FeSO
4
.7H
2
O (iron ions as trace elements of microbes). Because phosphates and magnesium salts are mixed to produce precipitation, in the mixing of culture base ingredients, the ingredients are generally dissolved in the order of the formula. For trace ingredients such as FeSO
4
.7H
2
O, a high concentration of storage fluid can be pre-arranged to add a certain amount as needed in the preparation of the culture base.No. 1 culture base formula is as follows:soluble starch 20gNaCl 0. 5g KNO
3
1g K
2
HPO
4
·3H
2
O 0． 5g MgSO
4
· 7H
2
O 0． 5g FeSO
4
·7H
2
O 0． 01gagar
15-20gwater 1000mlpH 7. 4—7． 6 , equipmentsoluble starch, KNO
3
, NaCl, K
2
HPO
4
.3H
2
O, MgSO
4
.7H
2
O, FeSO
4
.7H
2
O, agar, 1mol/L NaOH, 1mol/LHCl;test tube, triangular flavon,
beech
, volume, glass rod, medium displodator, torque
balance
, horn spoon, high pressure steam sterilization pot, pH
test paper
(pH5. 5—9． 0), cotton, cow paper, marker pen, hemp rope, gauze, etc., the operation step 1. Weighing and melting First named soluble starch according to the formula, put it in a small beech cup, and use a small amount of cold water to turn the starch into a paste, and then add less than the required amount of boiling water, continue to
heat
, so that soluble starch completely dissolved. It is also called that the other components dissolve one by one. For trace ingredient FeSO
4
.7H
2
O can be added first with a high concentration of storage fluid, by adding 1g feSO
4
.7H
2
O in 100 ml of water. 01g/ml, then add 1 ml of 0. 01g/ml of storage fluid is available. After all medicines have been completely dissolved, rehydrate to the desired total volume. If a solid culture base is to be made, its melting process is the same as that of experiment 19.2． pH regulation, displodation, wrapping, sterilization and sterile examination with experiment 19., the experimental report thinking question(1) What is a natural culture base? What is a synthetic culture base?best way to add trace elements when making synthetic cultures with (2)? Why don't natural cultures need to add trace elements?