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Commonly used cultures and ingredients
Time of Update: 2021-01-23
Lactic-bacteria Medium I (Lactobacillus Medium I) Yeast extract (yeast paste): 7.5 g Peptone (protein ): 7.5 g Gglucose (glucose): 10 g KH2 PO4: 2 g Tomato juice (tomato juice): 100 ml Tween (Twain) 80:0.5 ml Distilled water (distilled water) 900 ml pH 7.0.
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Two whiplash staining methods for bacteria
Time of Update: 2021-01-23
(i) Experimental purpose: to learn the bacterial whiplash dyeing method (ii) experimental principle: bacteria whiplash is very fine, diameter is generally 10-20nm, only with electron scope can be observed.
The water seal method is to drop the bacteria on the ordinary slide, and then cover the slide, under a microscope to observe.
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120 common toxic substances and protective methods in biological laboratories
Time of Update: 2021-01-23
2. Amino acetic acid: inhalation, ingestion, skin absorption can cause harm.
Wear appropriate gloves and goggles, protective clothing and operate in the ventilation cabinet.
Foric acid: highly toxic, mucous membrane tissue, upper respiratory tract, eyes, skin has great damage.
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Microscopic direct counting of microorganisms
Time of Update: 2021-01-23
3. Shake the yeast suspension well, draw a little with a dropper, remove a small drop (not too much) from the groove on both sides of the platform in the middle of the counting plate along the lower edge of the cover glass, so that the bacteria suspension fills the counting area with the surface pressure of the liquid, do not make bubbles, and use absorbent paper to suck out the excess bacteria suspended from the trench.
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Screening for the separation of bacteria produced by industrial microorganisms (III)
Time of Update: 2021-01-23
(i) Dilution coating method the soil sample to ten times the grade difference, diluted with sterile water, take a certain amount of dilution of the suspension, applied to the separation of frate plate, after culture, grow a single bacterios, pick the required bacterios moved to the sloped culture base culture.
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Phage outbreak and response measures
Time of Update: 2021-01-23
what we're doing is E. coli's genetic engineering bacteria. Some time ago even three batches were infected with phage , one batch a day, we do is 60T fermentation tank. of phage outbreaks: 1, PH
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The special dyeing technique of bacteria - spore staining film dyeing whiplash dyeing
Time of Update: 2021-01-23
Wash the excess dye with water, gently use absorbent paper to absorb moisture, dry mirror inspection, the result can be seen spores are dyed red, the bacteria appear blue.
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The method of virus isolation
Time of Update: 2021-01-23
The separation of viruses with cells is probably this process, of course, some viruses may not have the corresponding generation or primary cells, if you want to isolate can only use susceptible animals, operations are similar, nothing but sampling, sample processing, inoculation, post-vaccination detection and observation, collection of viruses only.
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Anti-acid dyeing principle and dyeing steps
Time of Update: 2021-01-23
anti-acid dyeing principle: Mycobacteria cell wall contains more lipids, of which the main component is mycobacteric acid, this material has anti-acidity, dyeing with charcoal acid redness binding
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Extraction of the total RNA of fungal mycelium
Time of Update: 2021-01-23
The RNA extract was preheated in a 65-degree C water bath before the experiment began, and ME (-based ethanol) was added to the centrifugal tube (10mL plus 80ul, 50mL with 300ul).
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Flat dash separation method
Time of Update: 2021-01-23
At the same time, with a ring-shaped inoculation needle in the flame to take a little bacteria suspension, quickly into the Petri dish, on the side of the plate culture base, for the first parallel dash 6 to 7, turn the Petri dish about 70 degrees angle, with the burned cooling inoculation needle, through the first dash section for the second parallel dash, and then use the same method, for the third parallel dash.
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Types of microbial aerosols in the laboratory
Time of Update: 2021-01-23
Both types of microbial aerosols are highly harmful to laboratory staff, depending on the toxicity of the microorganisms themselves, the concentration of aerosols, the size of aerosol particles and the micro climatic conditions in the laboratory at the time.
, Kenny and others have used Andersen samplers to determine the size of microbial aerosol particles produced during laboratory operations.
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A summary of the experimental experience of virus packaging
Time of Update: 2021-01-23
In doing virus packaging experiments, some researchers often encounter: with the same virus carrier system for virus packaging experiments, why do some people do very well, the second success, some p
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Bevel inoculation method
Time of Update: 2021-01-23
Fourth, with the small finger of the right hand and palm between the two test tubes on the cotton plug, while quickly burning the test tube on the alcohol lamp for about 3 seconds, so that the tube mouth may be contaminated with a small amount of bacteria to burn to death.
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Bacterial bio-chemical test - stone milk test
Time of Update: 2021-01-23
milk coagulation is mainly caused by bacteria containing lactase, which converts casein into a side casein, or is caused by the precipitation of casein by producing large amounts of acid from lactose fermentation.
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Identification of enterococcus genus
Time of Update: 2021-01-23
coli) Data statistics , among the pathogenic bacteria causing urinary tract infections, enterococcal infections ranked second celiac pelvic infections (E.
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Preparation of seed culture fluids and preparation of fermentation tanks
Time of Update: 2021-01-23
Glyceroid or bubble enemy" I, "> media preparation1, LB media Tryptrypt one 10g/L yest extract 5g/L NaCl 10g/L add distilled water to 100mL, 1 mol/L NaOH adjusts pH to 7.0,121C autocultensive 20min.
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The new kerstar of irritable bowel syndrome (IBS) - probiotic-assisted therapy
Time of Update: 2021-01-22
test methodChinese 45 IBS patients were randomly divided into control group (21) and probiotic group (24) at the Navy General Hospital of the People's Liberation Army in Beijing.
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Zhang Peace, Wei Wei, etc.: Early life environmental exposure affects the development of intestinal bacteria
Time of Update: 2021-01-22
Gut Microbes (IF:7.74) Exposure to soil environments when earlier life stages is distinguishable in the early stages of life when exposed to the soil environment in the early stages of life can distinguish the intestinal bacteria of adult mice 10.1080/1080/1 19490976.2020.1830699 2020-12-31, Article Abstract: Collected Environmental exposure exposure life stages can govern the assembly and development of the gut microbiota, yet it is insufficiently understood.
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Keeping "a valuable legacy on the tip of the tongue"
Time of Update: 2021-01-22
Original title: Inner Mongolia Dairy Biotechnology and Engineering Key Laboratory - Preservation of "Precious Heritage on the Tip of the Tongue Researchers went to the Tibetan herdsmen's families in
