Bevel inoculation method

method
microbial
from one slope
culment
bill to another slope
flevel
is called slope inoculation. Here's how:first, light the alcohol lamp. Place the
strain
and the two
tetry tubes
of the slope medium parallel to the left hand, clamp the thumb with the other four fingers, and place the middle finger between the two test tubes, the tubes of the two test tubes are flat.Second, use your right hand to screw the cotton plug loose and pull it out a little bit to help you pull it out during vaccination.Third, the right hand holding the vaccination needle, in the alcohol lamp will be the needle . All sections above the needle that may enter the test tube during vaccination are burned.The following actions should be done to keep the test tube port close to the fire.Fourth, with the small finger of the right hand and palm between the two test tubes on the cotton plug, while quickly burning the test tube on the alcohol lamp for about
3 seconds, so that the tube mouth may be contaminated with a small amount of bacteria to burn to death.Fifth, the burned inoculation needle into the bacteria tube, first of all, the needle contact the media on the area without bacteria (such as the top of the slope), so that it cools down, so as not to burn the inoculated bacteria. Then gently touch the bacteria, take out a little, slowly draw the inoculation needle out of the test tube, the inoculation needle pull out do not make the needle part hit the pipe wall and tube mouth, after removal, do not make the needle through the flame, not to touch other unrelated items or desktop.Sixth, quickly put the vaccination needle into another test tube, gently draw a line on the media, inoculated bacteria on it. When you draw a line, draw a dense parallel line from the bottom all the way to the top to make the most of the bevel area, but do not cut the culture base.Seventh, burn the test tube and plug the cotton plug. When stuffing, do not move the test tube to meet the cotton plug, so that the test tube in the movement of air that may contain bacteria.Eighth, burn and sterilise the needle on the flame. When you put it back in place, free your hands and stuff the cotton plug.