Types of microbial aerosols in the laboratory

operations in the laboratory can produce
microbial
aerosols and contaminate the laboratory's air as the air spreads, causing laboratory-related infections when staff suck up contaminated air. In pathogenic microbial laboratories, the resulting microbial aerosols can be divided into two categories: one is droplet nuclear aerosols, the other is dust aerosols.
Both types of microbial aerosols are highly harmful to laboratory staff, depending on the toxicity of the microorganisms themselves, the concentration of aerosols, the size of aerosol particles and the micro climatic conditions in the laboratory at the time. The study found that droplets with particle size of 100/xm sedent quickly, while droplets with particle size of <50 to m spread within 0.4s; droplet nuclei with particle size <5/xm can reach the vecups deep in the lungs after being sucked; and droplet nuclei with particle size >5/lm can be captured by the mucous membranes of the respiratory tract.
, Kenny and others have used Andersen samplers to determine the size of microbial aerosol particles produced during laboratory operations. It was found that the particle size <5 to m accounted for more than 98% of the aerosol particles produced by the stirring crusher. Among the aerosol particles produced by freeze-dried culture, the particle size >5/xm accounted for 80%. Other operations such as charging chicken embryo
culture
liquid,
straw
blow-absorbing mixing, centrifugation, ultrasonic crushing, shredding bacteria bottles, etc. produced by the average particle size of microbial aerosol particles are <5/Am.
generally speaking, the more microbial aerosol particles, the smaller the particle size, the more suitable the laboratory environment for microbial survival, the greater the likelihood of laboratory infection.