Commonly used cultures and ingredients

1. Acetobacter Medium (Acetobacter Medium
Culture
bicide)
Glucose): 100 g
Yeasst extract (
Yeast
Paste): 10 g
CaCO3: 20 g
Agar (
agar
): 15 g
Distilled water (distilled water) 1,000 ml Adjust (tune) pH to 6.8.
scope of application: foul-smelling Bacillus acetate turbid variant.2. Nutrient Agar (nutritional gravy agar)
Pepton (protein ): 5 g
Beef extract (beef paste): 30 g
NaCl: 5 g
Agar (agar): 15 g

Distilled water (distilled water) Adjust (tune) pH to 7.0-7.2
(Note): When plyon of Bacillus, 5 mg of to MnSO4.H2O may be added . It is favorable to promote spore formation .
Scope of application: gas-producing gas bacillus, fecal-producing Bacillus, waxy spores, waxy spores, herculin spores, giant spores, poly sticky spores, dust spores, short Bacillus spores, hot fat spores, dried grass spores, deep black variants of Bacillus spores, Suyun gold spores, Suyun gold spores paraffin subsethics (green worms), Suyun gold spores Bacillus Gorsd Variants, Suyun gold spores inverted subseeds, ammonia-producing Streptococcus, Yellow Staphylococcus aureus, Glutamate, Beijing Lollipop, E. coli (E. coli), copper-green prosthetic monocytobacteria (Green Pussy), convex Fake monocytobacteria, fluorescent prosthetic monocytobacteria, curved false monocytobacteria, fake monocytobacteria, vine octacicobacteria, jaundia octacicobacteria, urea octaccococcus, Staphylococcus aureus, sports fermented monospora.
3. Azotobacter Medium (nitrogen-fixing bacteria
medium
)
KH2PO4: 0.2 g
K2HPO4: 0.8 g
mgS O4.7H2O: 0.2 g
CaSO4.2H2O:0.1g
Na2MoO4.2H2O: Trace (micro) )
Yeast axtract (yeast paste): 0.5 g
Mannitol (glycol): 20 g
FeCl3: Tract (micro)
Distilled water (distilled water): 1,000 ml
Agar (agar): 15g
Adjust (tune) pH to 7.2.
scope of application: nitrogen fixation bacteria, glial spores.4. Corn Meal Medium (cornmeal medium)
Maize flour (cornmeal): 5 g
Peptone (protein ): 0.1 g
Glucose (glucose): 1 g
Tap water (tap water): 1,000 ml.
s Note: Boil the development in autoclave at 121 degrees C for 1 h. distribute the medium into 18x.18 mm tubes , each contains 10 ml of the liquid , then autoclave at 121 degrees C for 1 h

. 15 pounds sterilized again for 15 hours. ) 5. Lactic-bacteria Medium I (Lactobacillus Medium I)
Yeast extract (yeast paste): 7.5 g
Peptone (protein ): 7.5 g
Gglucose (glucose): 10 g
KH2 PO4: 2 g
Tomato juice (tomato juice): 100 ml
Tween (Twain) 80:0.5 ml
Distilled water (distilled water) 900 ml pH 7.0.
scope of application: Plant Lactobacillus (Lactobacillus germ), hot lactic acid streptococcus6. Lactic-bacteria Midium II. (Lactobacillus media II.)
Lacto-casein peptone (cheese ): 10 g
Beef extract (protein ): 10 g
Yeast extract (Yeast paste): 5 g
Glucose (glucose): 5 g
Tween (Twain) 80:1 g
K2HPO4: 2 g
Na-acet Ate (sodium acetate): 5 g
Diamine citrate (diamine citrate): 2 g
MgSO4.7H2O: 0.2 g
MnSO4.H2O:0.0 05 g
Distilled water (distilled water) 1,000 mL pH 6.5-6.8
Application: Plant Lactobacillus (Bacillus germ)

7. Peotone Glucose Yeast extract Medium PGY (protein, yeast paste, glucose medium)
Peptone (protein ): 10 g
Yeast extract (yeast paste): 5 g
Glucose (glucose): 1 g
Distilled water (distilled water): 1 L L.8. Glycerol Agar (glycerin agar)
Peptone (protein): 5 g
Beef extract (yeast paste): 3 g
Glycerol (glycerol) Oil): 20 g
Top water (tap): 1,000 ml
Agar (agar): 15 g
pH 7.0-7.2.9. Rhizobium medium (root tumor medium) AS 9
Yeast eztract (yeast paste): 1 g
Soil eztract (soil leachate): 200 ml
Mannitol (glycol): 10 g

Agar (agar): 15 g
Distilled water (distilled water): 800 ml
pH 7.2
s Note: Soil extract: Suspend 50 g finely and dried gardon soil in 200ml of tap water. Autoclave at 121℃ for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minutes at 121℃ , then mixed with othringredients and distributed. (Soil leaching method: take 50 grams of soil, add water 200 ml, 15 pounds of cooking for 1 hour, after
filtration paper
filtration
after filling up to 200 ml of water.)
The scope of application: soybean root tumor bacteria (slow-life type), soybean slow-forming genoma bacteria, peanut root tumor bacteria, purple cloud English root tumor bacteria, soybean root tumor bacteria (fast-forming type), soybean root tumor bacteria, pea root tumor bacteria, root tumor bacteria, cyanobacteria, tianyu root tumor bacteria 10. Mannitol Agar (glycol agar)
Yeast extract (yeast paste): 5 g
Peptone (protein ): 3g
Mannitol (glycol): 25 g
Agar (agar): 15 g
Distilled water (distilled water): 1,000 ml.