Flat dash separation method

(1) take 1 gram of soil sample, add 99 ml of sterile water to the flame in a conical bottle, block the cotton plug, make a bacterial suspension for use.(2) Take a
culture
dishes placed on the
experimental table
, the left hand will open the petri dish a little, into the Petri dish melted nutrient solids
ficification base
10 to 12 ml, gently turn the petri dish, so that the culture base in the distribution is uniform, flat table, so that it condenses into a flat plate. Then at the bottom of the dish with crayons to divide A, B, C, D several areas. Several plate cultures should be made in a row.(3) Secure the bottom of the Petri dish in a tilted state with the m and ring fingers, and open the Petri dish slightly next to the flame.
At the same time, with a ring-shaped inoculation needle in the flame to take a little bacteria suspension, quickly into the Petri dish, on the side of the plate culture base, for the first parallel dash 6 to 7, turn the Petri dish about 70 degrees angle, with the burned cooling inoculation needle, through the first dash section for the second parallel dash, and then use the same method, for the third parallel dash.
, the surface of the plate culture base should be lowered to prevent germs from falling into the air. The inoculation needle should be at an angle of about 30 degrees from the surface of the plate. Do not touch the needle on the edge of the petri dish, nor cut the culture base.(4) After the line of the Petri dish poured in the warm place of about 28 degrees C for culture, to grow bacteria backward, identification of
microorganisms
groups, and according to the results of the mirror examination, to determine whether it has been isolated to pure
strains
. If the strain is pure, it can be transferred to a sloped culture base for further culture. Continuous dilution separation and flat-plate dashing must be carried out in sterile chambers or inoculation boxes.