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    Home > Biochemistry News > Microbiology News > Problems related to glass beads in yeast granulation extraction

    Problems related to glass beads in yeast granulation extraction

    • Last Update: 2021-01-23
    • Source: Internet
    • Author: User
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    1: In the extraction of
    yeast
    particles, there is a step to use glass beads, who can tell me the role of glass beads, and where can buy glass beads, expensive? Thank you!
    1: At high salt concentrations,
    DNA
    adsorbed to glass beads, which are absorbed into the DNA, are centrifuged, washed away with salts and debris, and then washed away and resealed and suspended in water or TE buffers. DNA recovery rate is high and non-degradable, simple, fast and convenient to operate.
    2: Glass Bead SIGMA has, you can ask the agent. You can also use snail enzyme lysis to break the yeast cell wall method.
    2: Can you tell me specifically about the steps of snail enzyme lysing? Thank you. Besides, do you know the role of glass beads? It should be adsorption of yeast chromosome DNA, not proton DNA. You have used no, it is not expensive, because our boss is relatively small, so in the purchase of drugs more cautious. In the way glass beads are used, other drugs are in place, it's almost glass beads. Thank you!
    3: Tells you a simple and practical way to extract yeast granules, the purity of which can be used to sequence and
    PCR
    。 You can give it a try.
    method of extracting
    chlorpyride
    fungus
    a group. Add 0.1g bacteria and 500ul extract to the 1.5ml Appendorf tube, oscillate to mix it well, then add 10%
    SDS
    100ul, chlorpyride 300ul, oscillate violently, making the intra-tube mixture milky. 50 degrees insulation 1h, every 10min oscillation mix, and then add 300ul 3mol/LNaCl (ph=5.2), mixing, ice bath 15min, 6000rpm, 15min, collecting on the clear, add equal volume of waterless ethanol, room temperature precipitation 20min, then there will be flocculation precipitation appears. 10000rpm, 15min, precipitated with 70% ethanol washed once, room temperature as much as possible volatile residual trace ethanol, but do not let the DNA completely
    dry
    , add 50ulTEbuffer dissolved DNA.
    : 0.1mol/LTris-HCL, PH=9.0 plus 0.04mol/L
    EDTA
    , PH-8.0
    the whole process may need to be purchased, but it is also very cheap, 500ml only 32 yuan. ^_^
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