Yeast culture method

: Now the local boss to raise
yeast
, I do not know much about yeast, I hope that good people send some yeast basic knowledge and yeast
culture
attention to the problem and
legisic base
preparation what, thank you!
: Yeast is a single-celled
fungus
known to have more than 1000 kinds of yeast. Yeast can reproduce asexually through germination or by forming cystic spores. Asexual reproduction is when the environmental conditions are suitable, from the mother cell to grow a bud, gradually grow to mature size and separation from the mother. When nutritional conditions are poor, some yeasts that can reproduce sexually form spores that germinate when conditions are right.
cultured yeast with the most suitable pH value of pH4.5-5.0. The most suitable growth temperature is generally between 28 and 30 degrees C.
I did the experiment is mainly brewing yeast, I do not know the owner to do that genus of bacteria. The cultures used are:
Yeast Full Culture Base YPD: Yeast
Extract
10g, Protein Chen 20g, Glucose 20g, fixed to 1L. Adding 1.5%
agar
powder becomes a fixed culture base.
SC (Synthetic Full Culture) culture base (for screening yeast
conversion
children): YNB (yeast nitrogen source without
amino acids
) 6.7g, glucose 20g, add the corresponding amino acids, fixed to 1L. Adding 1.5% agar powder becomes a fixed culture base.
due to glucose and amino acids, sterilization is a sterilization condition with 115 degrees, 30min.
liquid shaker usually shakes 24-30min at 30 degrees, it is 24-30min, solid culture generally at 30 degrees to 3-5 days.