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    Home > Biochemistry News > Microbiology News > Potassium cyanide (KCN) culture base

    Potassium cyanide (KCN) culture base

    • Last Update: 2021-01-23
    • Source: Internet
    • Author: User
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    10g
    sodium chloride 5g
    potassium phosphate 0.225g
    sodium phosphate 5.64g
    distilled water 1000mL
    0.5% cyanide Potassium solution 20mL
    pH7.6
    method
    will be in addition to potassium cyanide after the distribution of
    -burning bottles
    ,121 degrees C
    autocultension
    15min. put in the refrigerator to allow it to cool down sufficiently. Every 100mL
    culture
    -base add 0.5% potassium cyanide solution 2.0mL (last concentration of 1:10000), divided into 12mm×100mm sterilization
    test tube
    , each tube about 4mL, immediately with sterilization rubber plug plug tight, placed in the refrigerator at least two months. At the same time, non-potassium cyanide
    culture
    as a control culture base, install test tube backup.
    test method
    inoculated
    agar
    cultures in protein water to become diluted bacteria, and 1 ring was selected to be inoculated in potassium cyanide (KCN) culture. Another ring was selected to be inoculated in the control culture. At 36 ± culture 1 to 2d at 1 degree C, and observed the results. If there is bacterial growth is positive (not inhibited), by 2d bacteria do not grow negative (inhibited).
    : Potassium cyanide is a highly toxic drug, use should be careful, do not get contaminated, so as not to poison. Summer sub-packing of the culture base should be carried out in the refrigerator. The main reason for the failure of the test is that the seal is not strict, potassium cyanide is gradually decomposed, resulting in the escape of hydrogen cyanate gas, so that the drug concentration is reduced, bacteria growth, resulting in false positive reaction. Special attention should be paid to each link during the test.
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