The pattern of line bacteria was observed by cellopic agar flat dialysis culture

(a) Experimental purpose:
the individual form of the line-releasing bacteria
cultured
with linen bacteria
(ii) Experimental principle:
the observation of the natural growth of the individual form of the line-releasing bacteria is now multi-use celloprotein
agaride
dialysis culture method.
celloprotein has semi-permeable membrane characteristics, its light transmission and slide is basically the same, the use of celloprotein agar flat dialysis culture, can make the line bacteria grow on the celloprotein, and then the long bacteria of celloprotein cut small pieces, attached to the slide, with
scope
mirror can be seen to the natural growth of the individual form of the line bacteria.
can also observe the individual form of the line-releasing bacteria by using the chip culture method.
(iii) Experimental equipment:
1, live material: culture 5-7 days of purple straight silk chain mold (Streptomyces violaceorectus) slope
strains
, absorbent chain mold 5102 (Streptomyces hygroscopicus var. Yingchengensis (5102)) beveled bacteria.
2,
Media
: Gaussy No. 1 agar media (see appendix I, 10)
equipment: sterile flat dishes, celloprotein, 9 ml sterile water several, alcohol lamps, matches, vaccination rings, tweezers, glass scrapers, 1 ml sterile
sorptors
, scissors, slides, microscopes.
(iv) Experimental method:
(1) cut cello into petri dish size, with old newspaper compartments stacked after sterilization.
(2) to make a 10-3 spore suspension of the line-letting bacteria beveled bacteria.
(3) Melt the Gaussia No. 1 agar culture base and pour it into a sterile petri dish next to the flame, pouring about 15 ml per dish, to be solidified by the culture base, under sterile operation, the sterile cellophair paper cover on the agar plate is made into a celloprotein agar plate culture base.
(4) with 1 ml sterile straw to take 0.2 ml absorbent chain mold (5102) spore suspension, purple straight silk streptomycemic spore suspension was added to the two celloprotech agar plate culture base, and with sterile glass scraper applied evenly.
(5) culture the inoculated celloprotein agar plate at 28-30 degrees C.
(6) to 3 days, 5 days, 7 days, remove the flat dish from the greenhouse. In a sterile environment. Open the petri dish, separate the cellogram from the media with sterile tweezers, and place the small piece with sterile scissors on the slide and observe it under a microscope.
attached: chip culture method:
line bacteria insert culture is the line bacteria species into spore suspension (concentration of 10-2-10-3 as well), take 0.2 ml placed in a plate culture suitable for the growth of line bacteria On the base, the glass scraper is evenly coated, and then the sterilized cover glass is inserted obliquely into the solid medium, cultured at 28-32 degrees C, 3-5 days later, the cover glass is removed and placed on the slide for mirror examination, which can be seen in the natural growth of the individual form of the line bacteria.