Myosome CCU determination

Lilac garden netizens "garden wood" to do mycosm CCU, and do not know how to choose the concentration of this initial bacteria liquid, because of the lack of standard 0.5 wheat concentration, so more confused, read the relevant literature said to quantify it to 10 6 squares, but how quantitative?
netizen "ljksbs" question and answer: this I have done before, can only be diluted with double dilution method: take 12 sterile
test tubes
, each tube pre-added 1.8 ml mycostic selective liquid
culture
base, in the first tube to add 0.2 ml of bacteria to be measured, fully mixed from the first tube to add 0.2 ml of liquid The second tube, then mix well, suck out 0.2 ml to add the third tube, and so on, to the last tube, put in 30 degrees culture, the fixed value of the CCU to the last tube color change as, that is, if the fifth tube color changes to the last tube, this CCU is 10 of the 5th square / ml. If the color of the sixth tube changes to the last tube, for 10 of the 6th square / ml.
Because mycoma's liquid growth is observed by color change as growth imagination, its growing liquid
fob
is still clear and bright, but the color changes, unlike most bacterial liquids are cloudy, so quantitative OD values are not necessary. Of course, in theory, solid cfu flat pouring hair is also feasible, but mycoplibacteria is very small, general observation to be observed in a low-fold mirror, according to the researcher's subjective observation difficulty, prone to subjective errors. Therefore, the better quantitative method of myosome is simpler and more feasible than diluted CCU, and is also commonly used.