What are the advantages and disadvantages of NEB's M13 phage random peptide library and NOVAGE's T7 phage peptide library?

s good, everyone! I have a few questions about PHAGE DISPLAY that I would like to ask you. Could you tell me about the advantages and disadvantages of Ph.D
and
and M13 c dna and peptide library? Under what circumstances is the choice better? And the cDNA library principle of NOVEGEN's prefabriced human tissue, under what circumstances? I'm going to study whether proteins that interact with testicular tissue are used in NOVEGEN's prefabriced human testicular cDNA library. Finally, would you like to ask the agents of these libraries?
: M13KET7
host cells: ER2738BL21/BLT5403/BLT5615
phage release mode: M13 phage solubility, non-cracking host bacteria. It greatly simplifies the phage purification step in each round of the selection process, using only simple PEG precipitation method. T7 is lysed and the protein it shows does not need to be secreted. This advantage allows more sequences to be shown. But it's not conducive to purification.
fusion: M13 phage N-side is fusion with pIII protein and is not suitable for cloning cDNA, as oligo d(T)) in cDNA is often located downstream of the translation termination cocoon. The C end of the T7 phage is fused with the T7 gene 10 shell protein, which can be expressed and displayed even if the insert contains a termination cocoon.
fusion sequence size: M13 phage display peptide library for 7 peptides, 12 peptides and ring
7 peptides, <30
amino acids
; T7 phage display peptide library prefab: 11329 yuan;
product use: NEBM13 phage display peptide library can be used to locate
antigen
decision clusters, determine
protein
interaction points, identify enzyme substrates or inhibitors, Identification of subject activators or inhibitors, development of
peptide
drugs, development of new drugs, tumor therapy and vaccine manufacturing, a wide range of uses. T7 phage peptide libraries can be used for protein interactions (e.g.
hormone
,
antibodies
, subjects), enzyme analysis, enzyme suppression; nucleic acid-protein interactions, antigen-determining clustering, mutation analysis.
you can choose according to your experimental needs, funding or other conditions.
addition, add: the difference between
I, Ph.D random peptide bank and M13 cDNA peptide library:
1, phage display peptide library is based on a completely random peptide library sequence to New England Biolab Ph.D seven peptides, for example, is a linear random 7 peptide library, containing 2.8 x 10 of 9 square clones, even if not completely the vast majority of all possible random 7 peptide sequences, that is, 20 of the 7 squares.
2 and cDNA expression libraries are limited to naturally occurring protein sequences.
3, compared with the main advantages of phage display technology is easy to filter the larger capacity of the library, the diversity is greater than 10 of the 9 parties. Standard cDNA library screening, limited by the number of phage spots or the number of clones that can be cross-screened, usually can only reach 4 orders of magnitude 10.
II, how to choose:
selection library is based on: your goal is to identify the sequence that can be closely integrated with the target molecule, whether natural or synthetic, or just want to identify the target molecule's natural in vivo match sequence. So ph. D is more suitable for screening and identifying new noseds or determining the interaction points between two known proteins. Systems such as cDNA libraries or
yeast
hybridization may be more appropriate when
natural lids for a protein.
for your experimental purposes, it may be that the cDNA library is more suitable.