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How yeast species are identified
Time of Update: 2021-01-21
Can you also recommend books on yeast identification and how to get them?
The fast identification bars they produce can identify many different kinds of yeast relatively quickly, or you can refer to the methods and principles of yeast identification by BioMerrier.
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Detection of E. coli in water bodies
Time of Update: 2021-01-21
protein , beef paste, lactose, sodium chloride heating dissolved in 1000mL distilled water, adjusted pH of 7.2 to 7.4, and added 1.4 6% bromophenol purple ethanol liquid 1.0mL, fully mixed, divided into duhan's tube tation tube, each tube 10mL, 115 degrees C sterilization 20min.
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The separation and purification of phages
Time of Update: 2021-01-21
, purpose requirements 1. Learn the basic principles and methods separation, purification bacterphages and diseases. 2. Observe phage spots. II, Basic Principles Because phages are specialized par
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Bacterial DNA extraction scheme
Time of Update: 2021-01-21
CTAB/NaCl method extracts DNA with higher purity, fewer protein impurities, and long shelf life, and editors prefer to add the final concentration of 20 μg/ml RnaseA to step 5, so that there is no RnaseA contamination in the end.
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Yeast breaking liquid nitrogen grinding method
Time of Update: 2021-01-21
Pour out the powder, melt in the 37-degree water bath, add liquid nitrogen with a syringe, repeat the above steps, repeat 3 times.
High-speed (20,000 rpm, 4 degrees 30 minutes) centrifugation (centrifugation) to clear, such as more impurities, need to be more than a few times away, the target protein (protein) purification with E.coli.
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Salmonella testing
Time of Update: 2021-01-21
1 topic content and scope of application this article specifies the testing method of salmonella in food. this article is applicable to the inspection of aviation food. 2 Equipment and materials st
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The difference between 胨 protein and 胨 protein protein
Time of Update: 2021-01-21
It is used as the main raw material of < microbiology< "> culture-based, in the fields of antibiotics, pharmaceutical industry, fermentation industry, bio-chemical products and microbiology The dosing is large; different organisms require specific amino acids and peptides, so there are various proteins , which generally include animal proteins (casein, meat) and plant proteins (beans) for protein production.
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Aseptic operation in the laboratory
Time of Update: 2021-01-21
(ii) Sterile operation steps After the preparation is completed, the implants from the natural growth conditions, according to the above-mentioned culture material disinfection method after sterilization, put into the sterilized Petri dish, and then placed under the ultra-clean work table alcohol lamp flame, with sterilized scissors and other equipment for proper separation, cutting or other post-treatment standby.
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Simple staining of bacteria and Terran's staining
Time of Update: 2021-01-21
Because of the low electrical points such as bacteria green , when it is often negatively charged when it grows in neutral, alkaline or weakly acidic solutions, it is usually colored with alkaline dyes (e.g. melancholy, crystalline purple, alkaline redness, or peacock green).
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How to slow virus infection
Time of Update: 2021-01-21
For example, the following parameters should be optimized prior to a large-scale infection to determine the most appropriate condition for an experiment: (1) cell growth density (2) number of lyovirus (3) concentrations of penicillin (4) infection time 2.
3. Add viruses to cells: (1) (Wall cells): Discard the medium and add fresh medium containing coagulation amines.
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Big discussion: How to identify a completely new virus using existing technology?
Time of Update: 2021-01-21
the technology of the 1980s was classic, but then molecular biology was not developed enough, is there any progress in the method now? How can you use today's technology to identify new viruses? I've
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Vaccination, separation and culture of microorganisms
Time of Update: 2021-01-21
Vaccination only need to pull out the cotton plug next to the flame, the tube mouth through the flame, with sterile straw to absorb the bacteria into the culture liquid, shake well.
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A quick way to identify bacteria
Time of Update: 2021-01-21
The 16S rDNA gene is analysis has become a standard method for the identification of bacterial genus and plays an important role in the classification of bacteria.
Chromosomal DNA prepared by this method can be used as a template for 16S rDNA sequences of PCR amplification bacteria without any treatment.
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Comics -- graduate students
Time of Update: 2021-01-20
Love is a kind of encounter, but can not foresee no incurable pain, no incurable sinking, all lost, will come back in another way Don't worship brother, sister-in-law will be angry I will be more than a dozen different ways of death to stop the boss ideal life real life sometimes leaving does not mean the end, perhaps a new beginning!
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The separation and cultivation of anaerobic bacteria
Time of Update: 2021-01-20
1 Purpose 1.1 Understanding the growth characteristics of anaerobic microbials 1.2 Observing the morphological characteristics of anaerobic microorganisms (Bifidobacteria1.3 Mastering the roll tube separation of anaerobic microorganisms, Training and Counting Technology 2 Principles Currently, simple and effective techniques for cultivating anaerobic microorganisms include: anaerobic tank culture technology, anaerobic tank culture technology, anaerobic bag culture technology, Hengeter anaerobic roller tube technology.
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Virus air spot experiment question and answer
Time of Update: 2021-01-20
However, methyl cellulose to be configured long in advance, about 2 weeks, after high pressure into the refrigerator, before use sterile operation to add culture fluid, mixed into the refrigerator, after a day to take out, shake hard, so that cellulose to achieve a homogeneous state, sit until the blister completely disappeared after use.
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Virus RNA extraction method
Time of Update: 2021-01-20
, isothionate extraction method (this is a detailed step to mention the avian influenza virus for reference) 1, take 200ul samples plus negative control plus positive control to add 1.5 ml sterilizati
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Bio-chemical experiments on bacteria
Time of Update: 2021-01-20
(2) Method: Inoculate the bacteria to be tested in glucose phosphate protein water, incubate 48h to 96h at 35 degrees C, add 5 to 6 drops of methyl red indicator in 5 ml medium, immediately observe the results.
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E. coli, fecal coli and E. coli testing methods in food
Time of Update: 2021-01-20
3base and reagents laurel sulphuric acid Salt tryptoprotein monthly display (LST) broth, yellow lactose bile salt (BGLB) broth, EC broth, yihong mei blue agar (EMB), nutritional agar sloping noodles, tryptophan broth, MR-PV culture, Kor Ser'ss broth, crystalline azithromid agar (VRBA), Butterfield's phosphate buffer dilution, physiological saline, Terran's dyeing solution, Kovacs' indigo substring reagents, methyl red indicators, Voges-proskauer (V-P) reagents, 4 sample preparation 4.1 Representative samples taken in sterile operations are held in sterilized containers.
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pfu and phage titration determination
Time of Update: 2021-01-20
7. Add the infected cells to the upper agar culture tube at a preheater of 45 degrees C, mix quickly one tube at a time, and immediately pour on the LB/IPTG/Xgal plate at 37 degrees C preheat.
