How to slow virus infection

, materials and
reagents
1.6 cm
culture
(Fiosher).
2.In human or mouse cell line, the growth required for cell testing
a
.
3.Coagulation amine.
4.The right choice
antibiotics
., instrument
1.
Cell culture
box third, step
1.The method of lysovirus infection should be optimized according to different cell line and cell-based experiments. For example, the following parameters should be optimized prior to a large-scale infection to determine the most appropriate condition for an experiment:
(1) cell growth density
(2) number of lyovirus
(3) concentrations of penicillin
(4) infection time
2.Plant the appropriate density of cells in a 6 cm Petri dish, 6 ml per hole volume.
(1) wall cells: 1 day before transfeding cells
(2) suspended cells: transfed on the same day planted cells, media need to contain coagulation amine.
3.Add viruses to cells:
(1) (Wall cells): Discard the medium and add fresh medium containing coagulation amines. Alternatively, discard some of the cultures and supplement them with coagulation amines. Adjust the volume and concentration of coagulation amine, so that the final concentration of coagulation amine is 8 ug/ml.
4.Viral infection:
(1) incubate cells overnight.
(2) replace the culture base 24 h after infection. Discard the culture base and replace it with 6 ml of fresh culture. If antibiotic options are required, use fresh cultures containing antibiotics.
note that the concentration of penicillin should be optimized for each cell line;
5. Incubate the cells and replace the incubator every few days as needed (with antibiotics if needed). The length of incubation time depends mainly on the post-infection test.