-
What are the similaries and differences between the original nuclear organism and the initial stage of the translation of the uterine nuclear organism?
Time of Update: 2021-01-21
(3) translation starts with a large, small sub-base of the carb body separated first;
(4) requires mRNA and ammonia-tRNA to bind to a small sub-base of thernac?
(6) small sub-base combines mRNA with the starter tRNA before it can bind to a large sub-base.
-
Biometrics of the grading of antibiotics
Time of Update: 2021-01-21
example: if 8 groups containing 20 units per milliliter of standard antibacterial ring diameter total average of 17.
-
Yeast powder and glucose and protein 胨 high temperature sterilization will not react
Time of Update: 2021-01-21
asked: glucose and yeast and powder and protein together with high temperature sterilization will not react? : Glucose and tryptophan react at high temperatures Maillard reaction Reaction diagra
-
Preparation of cultured and sterile water
Time of Update: 2021-01-21
II, equipment triangular bottles, test tubes, beers, glass rods, balances, enamel cups, cartridges, aluminum foil, angle spoons, Dushi tubes, silicone plugs, electric furnaces Nutrition Agaride Culture, Lactose Bile Fermentation Culture, Lactose Fermentation Culture, Imogen Melan agar Culture, Potato-Glucose Agar Culture (Additional Antibiotics), Plate Count Agar, Laurel Sulphate Tryplin frozen broth, yellow green lactose bile salt broth, EC broth, high-salt tsaccharin agar, triglyceric agar, etc.
-
Bacterial drug sensitivity test method
Time of Update: 2021-01-21
test is characterized by multiple strains of MIC determination at the same time, the repetition of the results is better than the broth dilution method, and easy to find contamination or drug resistance cientide bacteria, is commonly used in the new drug certificate of foreign drug sensitivity test classic reference standards.
-
The uerotymut gene expression system
Time of Update: 2021-01-21
The use of expression system to express the purpose gene in mammalian cells is an important means to study gene function and its interaction.
, more and more attention has been paid to the use of the nucleo-expression system to express the protein of purpose.
-
The relationship between bacterial 16srRNA and 16srDNA
Time of Update: 2021-01-21
"molecular biology has led to a breakthrough in the study of intestinal microecology. The most studied at present is 16S rRNA. In recent years, foreign scholars have used different molecular biolog
-
Recombination virus amplification and purification
Time of Update: 2021-01-21
3. CsCl continuous gradient centrifugal purification: 50 ml centrifugal tube weighing 4.4g CsCl, adding 8 ml virus lysate on the liquid, mixed, volume of about 10 ml.
After balancing, centrifuge 90min (SW28 turntable) at 23000rpm at 4 degrees C, suction of the lower layer of blue-white virus belt with a syringe 4.
-
The permeability of the cell membrane
Time of Update: 2021-01-21
Understanding the entry of various substances into red blood cells Speed II, Small Molecule Cross-Membrane Transport Overview Biofilm transmetric penetration of small molecules includes water, electrolytes and non-electrolyte solutes.
-
Bacterial membrane staining
Time of Update: 2021-01-21
3. Equipment: slides, slide shelves, wipe paper, microscopes, etc.
1. Negative staining method: (1) production: take a piece of clean slide, add a drop of distilled water, take a small amount of bacteria into the water droplets mixed and coated.
-
Advances in the research of lysovirus and its carrier system
Time of Update: 2021-01-21
Using pre-in-body construction of a carrier that can express shRNA, and then packaging it into lyovirus particles that can express shRNA, can directly infect some difficult cells, not only convenient, but also can achieve the long-lasting inhibition effect of gene expression.
-
Bacterial culture is commonly used in the preparation of cultures and antibiotics
Time of Update: 2021-01-21
5, 2×YT culturer dissolves the following parts in 0.9L water: protein16 g yeast extract 10g sodium chloride 4ml if needed to adjust pH to 7.0 with 1NNaOH (-1ml), then fill the water to 1L.
-
Phage MOI value and its significance
Time of Update: 2021-01-21
MOI was later widely used in the study of virally infected cells, meaning the ratio of viruses to the number of cells at the time of infection.
The meaning of MOI at this time refers to the average number of active units per cell infected with the virus.
-
Characteristics, culture and diagnosis of Helicobacter helicobacteria
Time of Update: 2021-01-21
Among them, gastric mucous membrane bacterial culture is the most reliable method to diagnose Helicobacter helicobacterium, which can be used as a "gold standard" to verify other diagnostic tests, and at the same time, it can carry out drug sensitivity tests to guide clinical selection of drugs.
-
The determination of phage titrity
Time of Update: 2021-01-21
When the cells grow, microwave melts the top layer of agarose gel, divided into a sterile culture tube, each tube 3 ml.
6. In addition to a micro centrifuge tube containing bacterial cultures to a diluted phage, only 10 ml of dilution is added to a micro centrifuge tube, rapid vortex, and 1-5min is incubated at room temperature.
-
Preparation of PDA culture
Time of Update: 2021-01-21
master "> culture-based formulation the same kind of bacteria< a href> formulation, although diverse, must contain a variety of nutrients needed for the growth of mycelium, Moisture and suitable < a href"" > acid- degrees.
-
The separation and purification of microorganisms
Time of Update: 2021-01-21
, the purpose requires master the method of inverting the plate and several commonly used basic operating techniques for separating purified materials.
Diluted coated plate method (1) inverted plate: add the paste protein agar medium, Gaussi I.
-
Common methods of bacterial culture culture
Time of Update: 2021-01-21
formula three tumor bacteria culture base glucose 10 g hydrophosphate d potassium 0.5 g calcium carbonate 3 g magnesium sulfate 0.2 g yeast powder 0.2 4 g agar 20 g water 1000 ml 1% crystalline azithy solution 1 ml first bring agar and water to boil and dissolve, then add the other ingredients separately, stir to dissolve, sub-pack, sterilize, spare.
-
Utensils commonly used in microbiology laboratories
Time of Update: 2021-01-21
(2) Glass straw sucks blood, serum, sugar solution or dye solution, such as glass straw (including capillary straw), after use should be immediately put into a tube containing tap water or specimen bottle, so as not to dry after difficult to rinse clean.
-
Standard strain verification and identification procedures
Time of Update: 2021-01-21
1.2 White Candida: picks part of the dry powder dissolved in a 0.9% aseptic sodium chloride solution, and lines are inoculated in improved Martin agar href The cells are egg-round, much like yeast bacteria, 5 to 6 times bigger than Staphylococcus alias, Glucose stain positive, but uneven coloring.
