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    Home > Biochemistry News > Microbiology News > Common methods of bacterial culture culture

    Common methods of bacterial culture culture

    • Last Update: 2021-01-21
    • Source: Internet
    • Author: User
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    commonly used bacteria
    culture
    -based culture method
    formula one beef paste
    agar
    culture base
    beef paste 0.3 grams, protein 1.0 grams, sodium chloride 0.5 grams, agar 1.5 grams,
    water 100 ml
    add 100 ml of water to the
    beabus
    , put in beef paste, proteinand sodium chloride, mark the outside of the be cup with crayons, put on a fire
    heat
    . After the ingredients in the beo bowl have dissolved, add agar and stir constantly to avoid sticking to the bottom. After the complete dissolution of agar to fill the water loss, with 10% hydrochloric acid or 10% sodium hydroxide to adjust the pH to 7.2 to 7.6, divided into the test tubes, with cotton plugs, with high-pressure steam sterilization for 30 minutes.recipe two potato culture base
    take fresh bovine heart (remove fat and blood vessels) 250 grams, with a knife finely chopped into mince, add 500 ml distilled water and 5 grams of . Mark the berries, bring to the boil and simmer over a simmer for 2 hours.
    filter
    , filtered meat
    dry
    treatment, filter pH to about 7.5. Add 10 ml broth and a small amount of minced dried bovine heart to each test tube, sterilise and spare.formula three tumor bacteria culture base
    glucose 10 g hydrophosphate d potassium 0.5 g
    calcium carbonate 3 g magnesium sulfate 0.2 gyeast
    powder 0.2 4 g agar 20 g
    water 1000 ml 1% crystalline azithy solution 1 ml
    first bring agar and water to boil and dissolve, then add the other ingredients separately, stir to dissolve, sub-pack, sterilize, spare.
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