Preparation of cultured and sterile water

, the basic principle
there
many kinds of culture base, different
microorganisms
need
the
different. The physical state after the foundation can be divided into liquid, solid and semi-solid. The cultures used at present are all biologic reagents
,
sheets.
II, equipment
triangular bottles, test tubes, beers, glass rods,
balances
, enamel cups, cartridges, aluminum foil, angle spoons, Dushi tubes, silicone plugs, electric furnaces

Nutrition
Agaride
Culture, Lactose Bile Fermentation Culture, Lactose Fermentation Culture, Imogen Melan agar Culture, Potato-Glucose Agar Culture (Additional Antibiotics), Plate Count Agar, Laurel Sulphate Tryplin frozen broth, yellow green lactose bile salt broth, EC broth, high-salt tsaccharin agar, triglyceric agar, etc.
4, method step
1, preparation of media:
(1) weighing: according to the media Formula, said to take the appropriate amount of medicine in the enamel cup;
(2) dissolved: the amount of water required by the amount of water, set the electric furnace
heating
, stirring, until completely dissolved, heated to boiling, unplugged, to be cooled;
(3) Sub-packing: according to different needs, immediately while hot into the triangular bottle or test tube, the triangular bottle to no more than half of the triangular bottle is appropriate, sub-packed test tube is generally 1/5 of the length of the tube (depending on the size of the test tube). Liquid cultures such as lactose bile salt fermentation cultures are about 10 ml in part.
(4) plug silicone plug: a test tube loaded with a culture base should be plugged with silicone plug, loose and suitable, close to the pipe wall, no gap, about 1/2 plug in, so that you can
filter
air, avoid clutter intrusion, but also slow down the evaporation of culture base moisture.
(5) packaging: triangular bottle cotton plug head applied tin foil wrapping, test tube focused on test tube.
2, sterile water preparation:
(1) with 10 ml pipe volume of 9.2 ml distilled water (diluted water) into the test tube.
(2) to take 95 ml of distilled water (diluted water) from a 100 ml barrel into a 150 ml triangular bottle. A small glass bead can be placed in a triangular bottle to prevent boiling.
(3) take 240 ml of distilled water (diluted water) in a 250 ml triangular bottle, plug the bottle with verse paper wrapped, autocultic backup.