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    Home > Biochemistry News > Microbiology News > Standard strain verification and identification procedures

    Standard strain verification and identification procedures

    • Last Update: 2021-01-21
    • Source: Internet
    • Author: User
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    species active:dry powder standard strain standard storage strain working

    ; 1.1 Standard strains of Staphylococcus aureus, E. coli, copper-green fake monocytobacteria, dead grass spores:
    pick part of the dry powder dissolved in 0.9% sterile sodium chloride solution, classified The line is inoculated on a < a href> tablet, 36 degrees C±1 degrees C < a href" > culture18-24 hours. And observe the pattern of the bacteria.
    a. Staphylococcus aureus: bulging, round, opaque;
    b. E. coli: colony slightly convex, round, moist, milky white;
    c. Copper-green false monocytobacteria: flat, round, untidy edges, smooth and moist, can produce blue-green or jaundice;
    d. Sporine spores: surface rough and opaque spores.1.2White Candida:
    picks part of the dry powder dissolved in a 0.9% aseptic sodium chloride solution, and lines are inoculated in improved Martin agarhref The cells are egg-round, much likeyeast bacteria, 5 to 6 times bigger than Staphylococcus alias, Glucose stain positive, but uneven coloring.
    black curvature: Pick part of the dry powder dissolved in a 0.9% sterile sodium chloride solution, line inoculated on an improved Martin agar slope medium, cultured for 48-96 hours at 23 degrees C-28 degrees C. The groves are dark brown and rough.

    1.2 Clostridium spores:
    pick part of the dry powder dissolved in 0.9% sterile sodium chloride solution, inoculated in sulfur ethanolate fluid medium 18-24 hours of culture from 30 to 35 degrees C, or 18-24 hours of anaerobic culture under anaerobic conditions in Colombia.2, species identification:
    2.1Staphylococcus aelobacter :
    Glucose staining test: Glucose-positive bacteria (purple), grape-like arrangement, coli.
    rabbit plasma test: inoculation of 1 monobacteria in 1 rabbit plasma solution, 30 degrees C-35 degrees C culture to 6 hours, every half an hour observation. Solidification is positive. Staphylococcus auspicrus is positive.

    2.2 E. coli:
    Gloranic staining test: Globacteria Glomere, red.
    imVC < a href" > bio:

    2.3 Copper-green prosthetic monocytobacteria:
    Glorene staining test: Globacteria, red A.
    oxidase test: the oxidase test paper was soaked with distilled water, with a fine glass rod or a disposable inoculation needle to pick out a single bacterium, applied to the test paper. Turning blue or blue-purple within 60s is positive and the invaricvity is negative.
    green pussycin test: take the slope culture inoculated on the PDP agar medium slope, culture for 24 hours, add 3 to 5 ml of chloromethane to the culture tube, crush the medium and shake fully. Set aside for a moment, move the chloromethane phase to another test tube, add 1mol/L hydrochloric acid test fluid about 1 ml, shake, sit for a moment, observe. If the hydrochloric acid solution is pink, it is positive for green pus, otherwise it is negative. The bacteria tested positive for green pus.
    fluorescence test: inoculated on a Kim's B culture (or a fluorescent pigment culture base produced by a fake monocytobacteria), cultured for 18-24 hours at 36 degrees C±1 degrees C, with blue-green fluorescence under a 366nm purple lamp.

    2.4 Bacillus spores:
    form: rough surface opaque, wrinkled.
    : stained with spore chromosomal, bacteria have spores. 2.5 White Candide:
    Glucose staining test: egg round, very much like yeast, 5 to 6 times bigger than Staphylococcus, Glucose stain positive, but uneven coloring.
    color-producing culture: 30-35 degrees C culture 24-48 hours, green bacteria.
    bud tube test: pick 1% Twain 80-corn agar media culture, inoculated with a drop of serum on the slide, covered with slides In the moist flat dish, placed 35 to 37 degrees C, 1 to 3 hours, placed microscope under observation, can be seen by spores grow short bud tube.

    2.6 : 48-96
    culture at 23C-28C. The plexus is dark brown, rough, and produced with black spores.

    2.7 Thorespora:
    Cleland staining test: Terratin-positive Thyrobacteria, with or without egg-free round or spherical spores.
    Colombian agar culture base test: inoculated on a Colombian agar culture plate containing gythromycin, cultured under anaerobic conditions for 48 to 72 hours, should grow.
    hydrogen peroxide enzyme test: drop plus 3% hydrogen peroxide test liquid, if there are bubbles on the surface of the bacteria produced, hydrogen peroxide enzyme test positive, otherwise negative. The bacteria is a negative reaction.

    3, need to buy reagents:
    < td style: "width: 469.0px;">HB4701 improved Martin agar culturer
    strains culture and reagents
    Staphylococcus acobacteria HB8278 Terrant staining liquid kit, HB4117 rabbit plasma, HB0109-7 nutritional agar culturer
    E. coli HBIG13 HBI E. coli bio-chemical identification bar, HB8280 methyl red kit, HB8281 V -P kit, HB8279 Kovacs indigo substitique kit
    copper-green fake monocytobacteriaHB5187 chlorpyure assay culture base (PDP), HB2100 oxidase test paper, HB8484 protomonitone fluorescent pigment
    withered grass sporesHB8300 spore staining Liquid
    White CandideHB701 5 Bacillus color-producing culture, HB0236-1% Twain 80-corn agar culture
    black
    Cyspora HB0124-1 Colombian agar culturer, HB0124-1a sulphate gydamycin (4mg/branch),
    HB8650 3% hydrogen peroxide test liquid
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