The uerotymut gene expression system

gene expression technology has
into
field of life science research since the birth of gene-based engineering in the 1970s. And with
implementation of
Human Genome Project, great progress has been made in technical methods, and remarkable achievements have been made to this day. With the completion
human
, more and more genes have been discovered, most of which do not function well. The use of expression system to express the purpose gene in mammalian cells is an important means to study gene function and its interaction.
in various expression systems, the earliest to be used for research is the primary nuclear expression system, which is currently the most mature expression system. The main method of this technique is to convert vectors (usually protons)

bacteria (usually E. coli) that have been cloned into the destination gene
DNA
segments, and to induce and eventually purify the desired end protein through IPTG. The advantage is that gene expression products can be obtained in a relatively short period of time, and the cost is relatively low. But at the same time, the primary nuclear expression system also has many difficult to overcome shortcomings: such as the usual expression system can not regulate the expression time and expression level, the continuous expression of some genes may have a toxic effect on the host cell, excessive expression may lead to non-physiological reactions, the purpose protein is often expressed in the form of inclusives, resulting in product purification difficulties;
In order to overcome the above deficiencies, many scholars have introduced the primary nuclear gene regulation system into the field of theochemical gene regulation, the advantages of which are:
(1) according to the highly specific design of the action between prokeruclear bioprotein and target DNA, and the target DNA and the ethonuclear gene regulation sequence are basically undecent, so there is no non-specific activation or inhibition of genes;
(2) can induce the efficient expression of genes, up to 105 times, which is not possible for other systems,
(3) can strictly regulate gene expression, that is, not only to control the "switch" of gene expression, but also to artificially regulate gene expression., more and more attention has been paid to the use of the nucleo-expression system to express the protein of purpose. At present, the commonly used eneromic expression systems in genetic engineering research are
yeast
expression system, insect cell expression system and mammalian cell expression system.
the U.S. ethyrnel gene expression system
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